膜技术分离纯化茶多糖工艺研究.PDFVIP

安徽农业大学学报, 2015, 42(1): 12-17 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x032 网络出版时间：2014-8-27 16:21:21 [URL] /kcms/doi/10.13610/ki.1672-352x032.html 膜技术分离纯化茶多糖的工艺研究 张 艳，杜先锋* (安徽农业大学教育部茶叶生物化学与生物技术重点实验室，合肥 230036) 摘 要：茶多糖是一类具有生物活性且结构复杂的糖蛋白复合物。结合膜集成联用技术通过微滤和超滤分离方 法对粗制茶多糖溶液进行纯化，同时对纯化后的茶多糖进行结构和活性分析。结果表明，以 0.05 μm 的膜孔径，0.2 MPa 的操作压力和 1%的料液为最佳微滤工艺条件，再经 30 kD 的超滤膜进行二次纯化，多糖含量从 50%提高至 81%，工艺简单易行，适宜于工业化生产，同时保存多糖的活性。 关键词：茶多糖；微滤；超滤 中图分类号：TS272; Q539 文献标识码：A 文章编号：1672−352X (2015)01−0012−06 Separation and purification of tea polysaccharides with membrane technology ZHANG Yan, DU Xianfeng (Key Laboratory of Tea Biochemistry Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036) Abstract: Tea polysaccharides are a group of glycoprotein compounds that have biological activity and com- plicated structure. In this study, we combined microfiltration with ultrafiltration separation methods to purify the crude tea polysaccharides. The structure and activity of the purified polysaccharides were analyzed. Results showed that the optimal processing conditions of microfiltration for tea polysaccharide purification were: using the membrane with pore size of 0.05 μm, under 0.2 MPa operating pressure with 1% feed concentration of the crude solution, followed by the second purification through a 30 kD ultrafiltration membrane. The yield of poly- saccharides increased from 50% to 81% using this purification method. In conclusion, this method is simple and the purified polysaccharides remain high activity; thus this method is suitable for industrial production. Key words: tea polysaccharide; microfiltration; ultrafiltration 茶多糖是从茶叶中提取出来具有多种生物活性