生物化学 chap2 Protein 1youngyu教材编辑.pptVIP

Assignment for chapter 1;Professional terms;amino group (-NH2) 氨基carboxyl group （-COOH）羧基 hydrophobic 疏水的 aromatic 芳香基团的 aliphatic 脂肪族的 polar 极性的 charged 带电荷的 uncharged 不带电荷的;Main contents;Section 1 Chemical compositions;Meaning of 6.25;For example If the N of a protein sample is determined to be 3.0 grams, calculate the crude protein concentration of the sample. Key: 18.75 g Crude protein / %=N%?6.25;Section 2 amino acids (AA); (1) A primary amino group (-NH2) and a  carboxyl group (-COOH)(2) ?-carbon atom(3) if R=H, glycine(4) D-,L-amino acid;2.2 Classification of AA;Hydrophobic, aliphatic R group;Hydrophobic, aromatic R group;Polar, charged R group;Polar, uncharged R group;Professional terms;nucleotide base 核酸碱基 helix 螺旋 pleated sheet 折迭 parallel / antiparallel 平行的/ 反向平行的 coil / loop 卷曲、环 globular protein 球蛋白 fibrin 纤维蛋白 biologically active 具有生物活性的 hydrogen bond 氢键 hydrophobic interaction 疏水相互作用;tertiary 第三的 quaternary 第四的 three-dimensional conformation 三维空间构象 electrostatic force 静电力 salt bridge 盐键 strength and rigidity 强度与刚性 spatial 空间的 amphoteric 两性的 ;2.3 Ionization(解离); Isoelectric point (pI)（等电点）: the pH at which AA has no net charge.(AA所带净电荷为0的环境pH) Neutral amino acid: pI=1/2(pK1’+pK2’);Acidic amino acid: pI=1/2(pK1’+pK2’);alkaline amino acid: pI=1/2(pK2’+pK3’);Several special amino acid (pI);Dissociation curves of amino and carboxyl group;For example;Section 3 protein structure;3.1 Primary structure; oligopeptide (up to 25 amino acid residues) polypeptide (25 amino acid residues); ? Peptide chains are written down with the free ?-amino group on the left, the free ?-carboxyl group on the right and a hyphen between the amino acids to indicate the peptide bonds.e. g. H2N-serine-leucine-phenylalanine-COOH Ser-Leu-Phe / S-L-F;Definition The primary structure of a protein is the linear sequence of amino acids as joined together by peptide bonds(氨基酸通过肽键连接而成的链状结构) (1)peptide bonds (肽键)