肌萎缩侧索硬化脊髓内源性神经前体细胞的变化及其调控因子HoxB表达改变的研究-神经病学专业论文.docxVIP

Abstract Abstract 万方数据 万方数据 ABSTRACT Objectives: Using mutant G93A-SOD1 transgenic mice as the animal model of mimicking human ALS.Observed and analyzed the distribution, proliferation,differentiation and migratory pathways of NPCs and the expression of HoxB9 in the adult mouse spinal card under physiological and ALS pathological conditions. Methods: Adult G93A-SOD1 C57BL/6J transgenic mice were used in our experiment, detection and screening of transgenic mice by PCR.We adopted fluorescence immuno histochemical double-labeling technique to label NPCs, neurons, astrocytes, oligodendroglia cell