肌醇六磷酸对裸鼠皮下SKOV3细胞移植瘤的抑制作用及其机制研究-妇产科学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要············································································································1 英文摘要············································································································4 研究论文 肌醇六磷酸对裸鼠皮下 SKOV3 细胞移植瘤的抑制作用及其机 制研究 前言·············································································································8 材料与方法·································································································9 结果··············································································································15 附图··············································································································17 附表···············································································································21 讨论··············································································································26 结论··············································································································30 参考文献·····································································································31 综述 肌醇六磷酸对多种肿瘤抑制作用的研究进展·····································34 致谢······················································································································44 个人简历···············································································································45 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 肌醇六磷酸对裸鼠皮下 SKOV3 细胞移植瘤的抑制作用 及其机制研究 摘 要 目的: 研究肌醇六磷酸（inositol hexaphosphoric acid ,IP6）对裸鼠皮 下 SKOV3 细胞移植瘤的生长、增殖抑制作用及其可能的机制。 方法： 1 细胞培养：将 SKOV3 细胞系置于含 10%新生牛血清的 RPMI- 1640 培养基中，在 37℃、5%CO2 的饱和湿度培养箱中常规培养。 2 裸鼠皮下 SKOV3 细胞移植瘤模型的建立与实验分组：将对数生长 期的 SKOV3 细胞接种于裸鼠的背侧近前肢处的皮下，于种瘤第 5 天选择 生长出移植瘤且直径在 3～5mm 的裸鼠入组实验，裸鼠随机分为对照组、 IP6 低剂量组、IP6 高剂量组、顺铂组及 IP6 低剂量+顺铂联合组，每组 6 只裸鼠。 3 实验药物的制备：IP6 和肌醇的混合物溶于 0.9%氯化钠注射液中， 配成含 IP6 为 3mg/ml、6mg/ml 的溶液；顺铂溶于 0.9%氯化钠注射液中， 配成 0.3mg/ml 的溶液，均现配现用。 4 给药方法：对照组按 0