近红外光谱法测定抗感泡腾片生产过程中绿原酸类化合物含量.pdf

·54· Chinese Journal of Information on TCM Apr.2011 Vol.18 No.4 近红外光谱法测定抗感泡腾片生产过程中 绿原酸类化合物含量 张媛媛,张加晏,迟玉明,杨光,叶祖光 (中药复方新药开发国家工程研究中心,北京 100075) 摘要：目的 建立抗感泡腾片生产过程中绿原酸类化合物的在线含量测定方法。方法 以抗感泡腾片生产工艺 为基础,在线收集样品,采用分光光度法,以绿原酸为对照品,测定样品中绿原酸类化合物的含量,同时应用傅立叶变 换近红外光谱仪透射光谱技术对样品进行检测分析,采用偏最小二乘回归法建立抗感泡腾片提取液中绿原酸类化合物含 量的近红外数学校正集模型。结果 近红外光谱中选择光谱区间为9 925.78～8 817.63/cm和8 593.25～7 449.53/cm。 所建立的模型相关系数 r 达到 0.969 48,校正均方差为 0.088 6。结论 本方法检测抗感泡腾片提取液中绿原酸类化 合物含量简捷、准确、快速,可用于抗感泡腾片生产过程中的在线质量控制。 关键词：近红外光谱法；抗感泡腾片；绿原酸；含量测定 DOI：10.3969/j.issn.1005-5304.2011.04.022 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2011)04-0054-03 Determination of Chlorogenic Acids Content in the Preparation of Anti-flu Effervescent by Near Infrared Spectroscopy ZHANG Yuan-yuan, ZHANG Jia-yan, CHI Yu-ming, YANG Guang, YE Zu-guang (State Engineering Research Center for RD of TCM Multi-ingredient Drugs, Beijing 100075, China) Abstract ：Objective To establish a on-line method for the determination of chlorogenic acids in the preparation of Anti-flu effervescent. Method Based on the production process of Anti-flu effervescent, online collection of samples, chlorogenic acids content in the sample was determined by UV spectrophotometry, with chlorogenic acid as the reference substance. While detected and analyzed samples was determined by Fourier transform near infrared spectrometer transmittance spectra, by means of partial least squares regression, calibration mathematical model for chlorogenic acids in aqueous solution of Anti-flu effervescent extract was established. Result The best spectral interval in NIR spectrum correlating with chlorogenic acids content