用蛋白质芯片联合检测卵巢癌患者血清的肿瘤标志物.pdf

用蛋白质芯片联合检测卵巢癌患者血清的肿瘤标志物 邓安梅 仲人前 陈孙孝 周 晔 孔宪涛 【摘 要】目的:应用蛋白质芯片联合检测卵巢癌患者血清中肿瘤标志物CA125、CEA、CA153和CA242。方法: 将血清与蛋白质芯片在37℃反应30min，再依次与反应液、检测液孵育，用生物芯片检测仪读取AFP、铁蛋白和 CEA反应点。结果:卵巢癌患者的蛋白质芯片检测阳性率为90%，显著高于正常对照、疾病对照组、蛋白质芯片 的反应强度与血清标本中CA125、CEA、CA153含量具有较好的相关性。结论:采用蛋白质芯片联合检测多种肿 瘤标志物，有助于提高肿瘤诊断的灵敏性。 【关键词】蛋白质芯片；卵巢癌；肿瘤标志物 【中图分类号】R737.31 文献标识码:A 【文章编号】100028578(2002)0620475202 United detection of tumor markers in Patients with ovarian cancer with protein chip DENG An-mei，ZHONG Ren-qian，CHEN Sun-xiao，et al Clinical Immunology Center，Changzheng Hospital，Shanghai 200003，China 【Abstract】Objective:In view of united detection of tumor markers such as CA125、CEA、CA153 and CA242 in patients with ovarian cancer with protein chip.Methods:The serum samples were incubated with protein chip at 37℃ for 30min,then incubated with reaction solution and detection solution.Finally,the reaction spots of AFP,Ferritin and CEA were read by biochip supplies analysis.Results:The positive ratio of C12 protein chip were 90% in ovaries cancer patients,which was markedly higher than those of normal and disease controls. The response intensity of protein chip was well correlated to the contents of CA125、CEA、CA153.Conclusion: United detection of tumor markers with protein chip is helpful for improving the sensitivity of tumor diagnosis. 【Key words】Protein chip；Ovarian cancer；Tumor marker 卵巢癌占妇科恶性肿瘤的第3位。由于卵巢癌深藏于盆腔，患病初期很少有症状，以致确诊时60% 70%的卵巢 ~ 癌已属晚期，其死亡率较高[1] 。寻找早期诊断方法，探索有效的综合疗法是降低卵巢癌死亡率的关键。但是， 肿瘤标志物的检测往往存在特异性和灵敏性不足的问题，联合检测有助于提高其诊断价值。 蛋白质芯片是一种高通量、高灵敏度、高特异性且微型化的蛋白质分析技术，是把多种纯化的探针蛋白高密度 排列在固定支持物表面上，样品中的靶蛋白可特异性地与探针结合，因而可对样品中的待测蛋白进行定性、定 量和功能分析[2,3] 。本实验采用肿瘤诊断用蛋白芯片试剂盒，对卵巢癌患者、正常人及疾病对照组血清中的多 种肿瘤标志物CA125，CA153，CA242和CEA进行联合检测，以便为临床上肿瘤的早期诊断和治疗提供更准确、 快捷的实验室检查依据。 1.对象和方法 1.1 血清样品取自 卵巢癌组：40例经临床病理确诊但都未经治疗的卵巢癌患者；正常对照组：159例年龄小于 30岁的