酚类化合物雌激素效应的比较研究-中国环境科学.PDF

中国环境科学 2009,29(8) ：873~878 China Environmental Science 酚类化合物雌激素效应的比较研究 1,2 1 1* 1 1 刘 芸 ,李 娜 ,马 梅 ,饶凯锋 ,王子健 (1. 中国科学院生态环境研究中心,环境水质学国家重点实验室, 北京 100085 ；2.环境保护部华南环境科学研究所,城市环境研究中心,广东 广州 510655) 摘要：采用重组人雌激素受体基因片段(hER)及共激活因子(GRIP1)双杂交酵母检测体系,检测 9 种酚类物质的类雌激素和抗雌激素效应. 结果表明,所检测的化合物中有 3 种同时表现出雌激素诱导与抑制活性;2 种只表现出雌激素诱导活性;1 种只表现出雌激素抑制活性;3 种均 没有雌激素诱导与抑制活性.结构分析表明:酚类中产生雌激素活性的物质具有取代基位于对位且邻位没有大的取代基团的结构;烷基在对 位的酚类物质也能显示出雌激素拮抗活性.所采用的双杂交酵母检测体系适用于外源物质的雌激素活性检测,具有较高的灵敏度;结合外源 物的雌激素诱导与抑制活性,用于分析污染物的体内作用方式. 关键词：酚类物质；类抗雌激素效应；双杂交酵母；结构分析 中图分类号：X171.5 文献标识码：A 文章编号：1000-6923(2009)08-0873-06 1,2 1 1* Comparative study of phenolic compounds estrogenic and anti- estrogenic activities. LIU Yun , LI Na , MA Mei , 1 1 RAO Kai-feng , WANG Zi-jian (1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085,China; 2.Metropolitan Environment Center, South China Institute of Environmental Sciences, Ministry of Environmental Protection of the Peoples Republic of China, Guangzhou 510655, China). China Environmental Science, 2009,29(8) ：873~878 Abstract ：The estrogenic and anti-estrogenic activities of 9 phenolic compounds were detected using recombined two-hybrid estrogenic receptor (hER) and co-activator (GRIP1) yeast assay. 9 detected phenolic compounds, 3 compounds had both estrogenic and anti-estrogenic activities; 2 compounds had estrogenic agonistic activities; 1 compound had anti-estrogenic activities; 3 compounds didn’t show any estrogenic or anti-estrogenic activities. The function of