抗铅菌株的筛选鉴定及其对铅污染麦田土壤修复效应-生态学专业毕业论文.docxVIP

AB AB STRACT In recent years，with the increasing of lead products and industrial‘three wastes”emissions，the state of the soil lead pollution is getting worse and worse．Heavy metal pollution is irreversible，when bioaccumulation exceeds certain limit，it will harm to humans．Traditional remediation methods of heavy metals pollution mainly physical and chemical methods such osmosis，ion exchange and chemical precipitation．But they would secondary pollution，high costS，energy consumption， operational difficulties and other problems．Fortunately microbial remediation has less pollution，less investment，simpler operation，which brought it to the attention of people．Thus，in order to provide theroitical basis and method for the remediation of Pb contaminated soil，two highly lead-resistant strains were screened and indentified from long-term heavy mental polluted soil Firstly,microorganisms play significant role in bioremediation of heavy metal contaminated soil and wastewater．In this stuay,two lead-resistant strains denoted by BY-1 0 and FB-6 were isolated from contaminated soil around lead refmery．And then biosorption characteristics of Pb2+of the strains were discussed，as well as the optimal growth conditions and identification ofthe strains．The results showed that， strains BY-1 0 and FB·6 belonged to Bacillus and Penicillium commune respectively，and the highest concentrations of Pb2+tolerated by them reached 1 700 mg／L and 2500 mg／L respectively．Concretely the optimal temperature of Bacillus cereus was 35℃and that of Penicillium commune Was 30℃：to both of them，the optimal growth pH was 7 and the liquid medium volume Was 80 mL-l 00 mL(in 250 mL bottle) With the initial concentration of Pb2+increasing from 0 mg／L to 600 mg／L，the growth of two strains became slower and slower gradually．And at certain concentration of Pb”，the adsorption rate of Pb2+by strains all increased firstly and then decreased gradually along with the growth time of strains．The dynamic change of biolog