绿色木霉的固定化技术研究-海洋生物学专业毕业论文.docxVIP

ABSTRACTCellulase ABSTRACT Cellulase is all important industrial enzyme，and Trichoderma viride produces hi曲 level cellulase．As we all known，Cellulase has the great ability of degrading cellulose．It is impotant to immobilize Trichoderma viride for producing cellulase，which probabily have a broad prospect． At present，the embedding method is mainly adopted to immobilize Trichoderma viride．The embedding method can effectively enhance the adaptability of Trichoderma viride against environment．It solves the problem of separating the fermentation liquid from Trichoderma viride and improves the cellulase activity．However，this method is expensive and the immobilized process is complex，what is more，the immobilized gel ball gradually becomes smaller and finally disappears诵tll the application time increase．So the embedding immobilized Trichoderma viride is difficult for large—scale industrial producting cellulase．In order to solve those issues，this paper studied the method of self-flocculation immobilization Trichoderma viride，and then optimized the fermentation medium for producing hi曲activity cellulase． 111e optimum conditions ofproducing green spore by Trichoderma viride in PDA solid medium is 1 0 mL of PDA culture medium in 1 00 mL Erlenmeyer flask．shading and 250C． Five days later,the green spores of Trichoderma viride was found distributing in the surface of the culture mediunl． The Trichoderma viride immobilization conditions were optimized by single factor and orthogonal test methods．The optimization conditions of self-flocculation immobilized Trichoderma viride Was pH 6．0，70 mL medium，2．25％glucose，1．50％peptone and immobilization time for six days．111e cellulase activity secreted by self-flocculating immobilization Trichoderma viride Was about 3．6 U，and the peptone dramatically affected the cellulase activity． After self-flocculation immobilization．the fermentation conditions for producing cellulase by Trichoderma viride was optimized by single factor,o