高糖对大鼠微血管内皮细胞基因表达谱的影响-中国科技论文在线.PDFVIP

上海医学2004年第27卷第12期 论著 · 高糖对大鼠微血管内皮细胞基因表达谱的影响 张斌 高鑫 李欣 【摘要】 目的 研究高糖对SD大鼠肺微血管内皮细胞基因表达谱的影响，进一步探索高糖导致微血管内 皮细胞功能紊乱的可能分子机制。方法 体外培养SD大鼠肺微血管内皮细胞，以Affymetrix公司的RG—U34A 基因芯片分别检测正常浓度(5．56 mmol／L)和高浓度(20 mmol／L)D一葡萄糖培养基中培养24 h后内皮细胞的基 因表达谱。结果 高糖培养细胞较正常浓度糖培养细胞有961个基因表达下调，509个基因表达上调，其中变 化幅度大于2倍的分别有22个(P0．998)和11个(P0．0025)，这些基因主要与细胞物质代谢、细胞信息传 递、细胞膜结构、细胞外基质合成及分解等有关。结论 高糖可能通过影响微血管内皮物质代谢、信号转导、细 胞膜结构和细胞外基质等的表达而导致血管内皮功能紊乱。 【关键词】基因表达谱；微血管内皮细胞；高浓度葡萄糖 Influence of high glucose concentration milieu on gene expression spectrum of rat pulmonary microvascular endothe- lial cell ZHANG Bin，GAO Xin，LI Xin． Department ofEndocrinology，Zhongshan Hospital，Fudan Uni一 ~rsity，Shanghai 200032，China 【Abstract】0bjective To observe the gene expression spectrum of pulmonary microvascular endothelial cells of SD rat in high glucose concentration culture media and tO investigate further the possible genes of microvascular en— dothelial cell which may be involved in the detrimental effect of high glucose resulting in microvascular endothelial dys— function．Methods Pulmonary Microvascular endothelial cells of SD rat were cultured in 5．56 mmol／L glucose(NG) and 20 mmol／L glucose(HG)media respectively for 24 h，and then RG—U34A gene chips from Affymetrix Corporation were applied tO test the gene expression of endothelial cells in the two groups．Results Comparing with gene expres— sion in NG group，the HG group had 33 genes expression which was 2 fotds greater，including down-regulation of 22 genes(Wilcoxon signed rank test，P0．998)and up—regulation of 9 genes(Wilcoxon signed rank test，P0．0025)． The functions of these genes mainly covered the cellular metabolism，structural components of extracellular matrix