小麦-黑麦附加系的创制及5R抗白粉病新基因的发现.PDFVIP

HEREDITAS (Beijing) 2011 11 , 33(11): 1158―1162 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2011.01158 小麦-黑麦附加系的创制及5R 抗白粉病新基因的发现 符书兰, 唐宗祥, 任正隆 , 611130 以重复序列pAS1 和pSc119.2 为探针, 对八倍体小黑麦×普通小麦的F5 代植株进行了FISH 分析, 同时对 这些材料进行了田间抗病性鉴定。从中鉴定出了 1R、2R 、3R 、4R 、5R 、6R 、7R 单体附加系和 1R、2R 二体 附加系, 1R 和4R 附加系出现频率相对较高。5R 和6R 单体附加系对白粉病免疫, 推测5R 染色体上带有新的白 粉病抗性基因。此外, 还检测到不少植株染色体组发生了变异, 且小麦4B 染色体优先缺失。 小麦; 黑麦; 附加系; 白粉病; 染色体缺失 Establishment of wheat-rye addition lines and De Novo powdery mildew resistance gene from chromosome 5R FU Shu-Lan, TANG Zong-Xiang, REN Zheng-Long State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang 611130, China Abstract: The F plants derived from octoploid triticale × common wheat were investigated by FISH methods using 5 repetitive DNA sequences pAS1 and pSc119.2 as probes. The disease resistance of these plants was also screened and evaluated in the field. The 1R, 2R, 3R, 4R, 5R, 6R and 7R monosomic addition lines and 1R and 2R disomic addition lines were found. The occurrence frequencies of chromosomes 1R and 4R addition lines were higher than that of chromosomes 2R, 3R, 5R, 6R, and 7R addition lines in the high generation screened. The 5R and 6R monosomic addition lines were im- mune to powdery mildew. The chromosome 5R in this study might carry new powdery mildew resistance gene(s). In addi- tion, the preferential elimination of chromosome 4B was observed in several plants. Keywords: wheat; rye; addition line; powdery mildew; chromosome elimination Pm7Pm8Pm17 Pm20 [6] Pm7 Rose, , 5%~10%, 2RL [7], Pm8 Pm17 Petkus 30%[1, 2