卵黄和低密度脂蛋白对冷冻保存绵羊精子胆固醇磷脂.pdfVIP

华北农学报# 2008, 23( 6) : 112- 115 郑云胜, 宏 伟, 倪利平, 田东海, 芦永跃, 邓常胜, 李金泉 ( , 010018) :, , LDL 9 , ,234. 54 nmol/ 10 9 163.76 nmol/ 10 , (c/ p) 0154 0136; 9 g/ 100mL LDL , 9 243.73 nmol/ 10 , c/ p 01 50,,c/ p ; LDL , , , c/ p : ; ; ; ; ; ; :S826 :A : 1000- 7091(2008)06- 0112- 04 Effects of Egg Yolk and LDL on Changes of Cholesterol and Phospholipids in Sheep Spermatozoa during Freezing ZH NG Yun-sheng,HONG Wei,NI Li-ping,TIAN Dong-hai, LU Yong-yue, D NG Chang-sheng,LI Jin-quan (Inner Mongolia Agricultural University,Huhhot 010018, China) Abstract:For search the reasons of low pregnant rate of sheep frozen semen, the paper studied on the changes of cholesterol and phospholipids in sheep spermatozoa during freezing, which was protected with egg yolk or low density lipoprotein(LDL) in diluents.The results indicated that the contents of cholesterol of sheep spermatozoa decreased from 9 9 234.54 nmol/ 10 sperm of fresh semen to 163.76 nmol/ 10 sperm of frozen semen and the ratio of cholesterol and phos- pholipids( c/ p) decreased also from 0154 to 0136when egg yolk was added in diluents.When egg yolk in diluentswas in- 9 stead with LDL 9 g/ 100mL, content of cholesterol in spermatozoawas 243.73 nmol/ 10 sperm after frozen and c/ p was also maintained 01501In conclusion,the content of cholesterol and c/ p in sheep spermatozoa decreased during freezing in egg yolk extender and LDL could prevent the content of cholesterol and c/ p from decreases. Ke