不同耐力训练量对兔股直肌肌球蛋白重链.docVIP

不同耐力训练量对兔股直肌肌球蛋白重链 论文: 不同耐力训练量对兔股直肌肌球蛋白重链 的影响 12 季苇，高维纬 5 (1. 北京体育大学运动康复系，北京 100084; 2. 北京体育大学运动医学教研室，北京 100084) 摘要:目的:研究不同耐力训练量对兔股直肌肌球蛋白重链各亚型相互转化的影响。方法: 45 只雌性新西兰大白兔，按耐力训练量的不同随机分为 G1、G2、G3、G4 和 G5 组。兔左 后肢作为实验侧进行耐力训练，右后肢为对照侧，无干预。各组完成规定运动量后，注射过 10 量戊巴比妥钠处死、取材、测定蛋白含量、聚苯乙烯凝胶电泳。用 Image J 图像处理软件对 电泳得到的图像进行分析。采用 SAS6.1.2 进行统计分析处理，显著性水平为 P0.05。结果: G4 实验侧 MHC IIb 相对含量较对照侧的显著减少(P,0.05);G5 实验侧 MHC I 相对含量 较对照侧的显著增加，实验侧的 MHC IIa 相对含量较对照侧的非常显著的减少(P,0.05，P ,0.01)。其它各组中未见实验侧与对照侧肌球蛋白重链各亚型相对含量存在显著性差异。 15 结论:固定运动强度，随着耐力训练量的增加，肌球蛋白重链各亚型之间的转化首先是以 MHC IIb?MHC IIa 为主，然后变为 MHC IIa?MHC I。 关键词:运动人体科学;耐力训 练;运动量;肌球蛋白重链;转化 中图分类号:G804.5 20 The effect of different endurance exercise amount on Myosin heavy chain of rabbit rectus femoris 12Ji Wei, Gao Weiwei (1. Sport Rehabilitation and Health Institute,Beijing Sports University, Beijing 100084; 2. the section of Sports Medicine, Beijing Sport University, Beijing 100084) 25 Abstract: Objective: The study was aimed at investigating the effect of different endurance exercise amount on Myosin heavy chain of rabbit rectus femoris. Methods: 45 adult female New Zealand rabbits according to different endurance exercise amount were randomly divided into G1,G2,G3,G4 and G5 group. Left legs were applied endurance exercise as the experimental side. Right legs were served as the control side. After finished the corresponding amount of endurance 30 exercise ,the rabbit were killed by using Pentobarbital sodium over-dose, the rectus femoris muscle were sampled, Protein content was tested by BCA method and MHC composition was assessed by using SDSprocedures. the pictures of MHC isoforms was analysed by Image J software. the statistical data were analysed by SAS6.1.2 and the level of significance was for P 0.05. Results: Compared with G4 control side, the proportion of MHC IIb decreased significantly 35 (P 0.05) ; the proportion of MHC I increased significantly (P 0.05) and the proportion of MHC IIa increased significantly (P 0.01)in G5 control side