固态酶型时间温度指示器性能研究.pdfVIP

第 38 卷 第 1 期 包 装 工 程 2017 年 1 月 PACKAGING ENGINEERING ·97 · 固态酶型时间温度指示器性能研究 唐园园，钱静 （江南大学，无锡 214122 ） 摘要： 目的 以糖化酶、淀粉为主要原料，研制一种激活方便的固态酶型时间-温度指示器。方法 对糖 化酶进行微胶囊化处理，以糖化酶反应体系为基础，研究不同因素对指示器色差值的影响，确定在 4 ℃ 条件下，时间在 5～6 d 的指示器配比。结果 确定了固态酶型 TTI 的反应配方，通过测定其在不同温度 下的反应速率，得到了 7 组 TTI 体系的活化能，其活化能值分别为 106.68, 98.47, 87.66, 89.73, 106.74, 97.51 和 87.99 kJ/mol。结论 得到可用于 4 ℃条件下的时间温度指示器的 7 组配方，根据其活化能的不同， 可用于指示不同产品的货架期。 关键词： 时间-温度指示器；淀粉；糖化酶微胶囊；色差值；琼脂 中图分类号：TB484.9 ；TS205.7 文献标识码：A 文章编号： 1001-3563(2017)01-0097-06 Characteristics of Time-temperature Indicator with Solid-state Enzyme TANG Yuan-yuan, QIAN Jing (Jiangnan University, Wuxi 214122, China) ABSTRACT: The work aims to develop a conveniently-activated time-temperature indicator with solid-state enzyme with glucoamylase and starch as the main raw materials. Glucoamylase microcapsules were prepared and the effects of differ- ent factors on the color difference of the indicator were studied based on the reaction system of glucoamylase. The for- mula of the indicator at 4 ℃ and within 5-6 days was determined. The formula of TTI with solid-state enzyme was de- termined ，and 7 groups of TTI system activation energies were obtained through measuring the reaction rate at different temperatures. The activation energy values were respectively 106.68, 98.47, 87.66, 89.73, 106.74, 97.51 and 87.99 kJ/mol. 7 groups of TTI formulas are obtained at 4 ℃. According to the different activation energies, they can be used to indicate the shelf life of different products. KEY WORDS: time-temperature indicator; starch; glucoamylase microcapsules; color difference; agar 近年来，随着经济的不断发展以及人们生活水平