苗药土党参化学成分及其生物活性研究-生物化学及分子生物学专业毕业论文.docxVIP

苗药土党参化学成分及其多糖生物活性研究中文摘要 苗药土党参化学成分及其多糖生物活性研究 中文摘要 关键词：土党参化学成分多糖生物活性研究 对土党参的化学成分进行研究。采用乙醇提取、溶剂萃取、大孔树脂吸附、硅胶柱 层析、凝胶柱纯化等从桔梗科植物土党参的根中提取分离单体化合物。通过波谱解析及 化学方法进行结构鉴定。分离了13个化合物，鉴定了其中的7个化合物，分别为：党 参苷I(Tangshenoside I，I)、丁香苷(Syringin，II)、5-羟基一4 7，6，7-三甲氧基 黄酮(5-Hydroxy一4’，6，7-Trimethoxyflavone，III)、5-羟基一4，’7一二甲氧基黄酮 (5一Hydroxy一4’，7一diTnethoxyflavone，IV)、蒲公英赛醇乙酸酯(taraxero!acetate V)、 无羁萜酮(friedelin，VI)和蒲公英赛醇(taraxerol，V11)。7个化合物均为首次从该植物 中分得。 采用DEAE纤维素(二乙氨基乙基纤维素)柱层析和Sephase凝胶柱层析分离纯化 土党参多糖，采用凝胶色谱法和纸层析法鉴定纯度，红外光谱测定其苷键类型。通过比 色法测定其多糖含量。分离得到的土党参多糖CJP一1含有半乳糖、甘露糖、葡萄糖，它 们的摩尔比为1：3．1：6．4，其分子量Mw为3．9233×103；CJP-2含有木糖、鼠李糖、 阿拉伯糖、甘露糖、半乳糖、葡萄糖，它们的摩尔比为1：1．6：3．7：1．7：6．4：14．0 其分子量Mw为7．2147；103。它们的红外光谱中有典型的多糖吸收峰，并具有糖苷键。 CJP．1及CJP一2为首次从土党参中提取获得，为土党参多糖的主要成分。 4 贵州大学2005届顽士学位论文AIbstract： 贵州大学2005届顽士学位论文 AIbstract： Key Words：Campanumoea javanica Chemical constituents Polysaccharide Bioactive Study To study the chemical constituents of Campanumoea javanica Compounds were extracted with alcohol，,distributed by different solvents．，,isolated via macroporous resin adsorbent and silica column chromat删y,punfied by silica gel chromatography．Their structures were identified by physicoehemical properties and spectral analyses．．Thirteen compounds were obtained from the roots of Campanumoea javanica and Seven compounds were identified Tang-shenoside(I)．,Syriln百n(H),5-Hyd】Iroxy-4,6：．7-Trimethoxy ftavorte(RI)，5-Hydroxy-4’,7-dimethoxyflavone(IV)，taraxerol acetate(V),,friedelin(VI)and taraxerol(vⅡ1．All ofthem isolated from Campanumoeajavanica for the first time． To study the isolation and composition of Polysaccharide of Campanumoea j￡tvanica BL．Polysaccharide(CJP一1，CJP·-2)CJPwaspurifiedbyDEAEand sepharosec．The component of the sacchride was determined by GC and TLC．Its MW was measured by gel filtration,．The form of glucosidicbond WRS detrermined by皿．The content of the saccharide was measured by colorimetry．CJP一1’S sugar composition comprises：Galactose，．mannoge，,glucose．With rate of 1：3．1：6．4．And its average MW WaS mensura