黄花蒿中提取与纯化青蒿素的研究-化学工程与技术专业毕业论文.docx

浙江大学博士学位论文℃。研究表明采用二次萃取的方式不能达到进一步提纯青蒿素的目的。根据小 浙江大学博士学位论文 ℃。研究表明采用二次萃取的方式不能达到进一步提纯青蒿素的目的。根据小 试结果，初步设计了超临界c02萃取青蒿素的生产工艺流程。 分别采用“热球”模型和基于质量衡算微分方程的模型来描述超l临界萃取 过程。在实验范围内，利用质量衡算微分方程的模型方程式计算的萃取率与实 验值能够较好地吻合，最大相对误差为14．8％，最小为1．2％。 在柱层析一结晶纯化青蒿素的研究中，选择硅胶作为柱层析吸附剂。30C 下硅胶对青蒿素的饱和吸附量为35．2 mg／g。以正己烷／乙醚=80／20(v／v)作为 流动相，青蒿素的Rf值为0．16。在尺寸为中2．0 cm X 50 cm的层析柱上，优 化得到较优的操作条件：流量1．5 ml／min，柱温为25C，上样量15．0 mg，g硅 胶。经过柱层析分离，青蒿素的纯度从12．25％提高到70％左右，收率在88．5 ％以上。吸附剂硅胶经溶剂(正己烷／氯仿=60／40(v／v))再生后可以多次重 复使用。柱层析得到的青蒿素粗品，用约17倍体积(mVg)的70％乙醇结晶， 得到青蒿素纯品，纯度为98．9％，结晶收率为85．2％。 完善了青蒿素提取、精制新的工艺路线：超临界C02萃取一柱层析分离一 结晶精制，青蒿素产品纯度为98．9％，总收率达到71％以上。 关键词：青蒿素，溶解度，超临界C02萃取，柱层析，结晶 Ⅱ 浙江大学博士学位论文Artemisinin,which 浙江大学博士学位论文 Artemisinin,which is isolated from artemisia annua三一is considered to be the most promising drug for treatment of malaria．It was developed in China and has a large demand．Conventional methods based on solvent extraction of artemisinin are time- consuming,energy-consuming and of low extraction yield since they require multifle extraction steps．Supereritieal C02 extraction is considered as a uow and green process for value-added natural products．The main objective of this dissertation is to study the process of extracting artemisinin with supercritical C02 and purifying the extracts．n垃new separation process of artemisinin,which includes supereritical C02 extraction,column chromatographic separation and crystallization,is expected to be applied commercially． The method for detection and quantification of artemisinin by reversed-phase HPLC has been developed．This quantitative method is accurate,sensitive，and reproducible， which is suitable to the analysis of artemisinin in crude plant extracts．The relative standard deviation is less than Ll％．The recovery is 98．9％． A flow-type apparatus has been built for solubility determination of solid in supereritical C02．The solubility ofartemisinin in supercritical C02 was measured in a pressure range of 10 MPa tO 27 MPa and a temperature range of 37℃to 65℃．The range ofsolu