酶和药物在聚电解质微胶囊中的自发沉积与可控释放-材料学专业毕业论文.docx

浙江大学顽士学蚀论文摘 浙江大学顽士学蚀论文 摘 要 采用屡一层组装法制备聚峨解质中空微胶囊在避年来引起了广泛的注意，但如何对 魏矮进行裔效灏包埋是这类聚锻释艨徽胶囊在生物技术、纯工、制药等领域实现其应用 靛一个熏袋必决条俘。赣近发疆鹣聚电解矮徽获囊巍爱掰其毒豹诱导耢溪垦发沉获酶往 质为物质的觎埋开创了一条崭新的思路。本文旨在研究以此方法对不同物质进行沉积包 埋及包埋尉的释放性能。 本文研究了过氧化物酶(HRP)的包埋特性和戗埋后的剩余活性及穗定性。HRP通 过塞发滠积蔽零被毽淫虱了徽羧囊孛；滋必共聚焦鬣徽镜、摹子力曼缀缓秘透鼓毫镜双 察证明了HRP的自发沉积。研究发现，沉积后的HRP活性损失很大，倔沉积后仍具有 活性的部分黼在高温、长时间和有机溶剂等环境下寝现出了明显好于游离HRP的催化 稳定性。 本文逐疆突了，I、分子模型药物(罗丹明B，RdB)帮遥黏抗癌药物(羰籀，Cisplatim 卡铂，Carboplatin；柔红霉素，DNR{阿霉素，ADM)在聚电解震徽胶囊中静鑫发滚积 和释放性能。激光共聚焦显微镜、原子力显微镜和透射电镜首先证明了以上药物沉积的 发生。在(1)不同初始浓度、(2)不同温度、(3)不同盐浓度和(4)不同pH值下对 模型药物Rhd B和拭癌药物DNR沉积和释放的研究发现高初始浓度、高温、高盐和高 龌整强蘸下骞裁予药鐾蕊滚获；嚣低裙始载藜虽、低滋、褰整霹舔p瓣德强凌下，滚瑷 后的药物则其有相对平缓的释敞性能。这是因为不阏环境下小分子药物秘具有自发沉积 性质的胶囊内的三聚氰胺甲醛／浆苯乙烯磺酸盐(MF／PSS)复合物之间的电荷作用、胶 囊内外药物浓度的平衡和药物自身溶解度等多重因索造成。 关键谣：蒙电解质馥皎囊，鑫发滚积，包埋，遗畿倔钫酶，藏瘸蓊糍 浙江大学硕士学位论文 浙江大学硕士学位论文 Abstract In recent years，polyelectrolyte multiplayer(PEM)microcapsules prepared by layer-by-layer adsorption of oppositely charged polyelectrolytes have received considerable attention．However,desirable substances should be encapsulated into these capsules at first in order to meet the different applications in the fields like biotechnology,chemical engineering and pharmacy．The novel finding that some PEM capsules Call introduce spontaneous deposition of various substances provides a brand-new way of encapsulation．The main objective ofthis thesis is to explore the deposition properties ofdifferent substances as well as t11eir release behaviors． First，the deposition properties of horseradish peroxidase(HRP)and its activity after deposition are investigated in this article．Confocal laser scanning microscopy(CLSM)， atomic force microscopy(AFM)and transmission electron microscopy(TEM)observations verified the occurrence ofthe deposition．After HRP deposition，majority of its initial activity was lost；However,the left part which still remains the activity has the hi曲resistance to denaturation environments like hi曲temperature，long incubation time and organic solvent exposllte- In this thesis，the deposition properties