补肾固齿丸对大鼠试验性牙周炎牙槽骨重建的影响-第三军医大学学报.DOC

补肾固齿丸对大鼠实验性牙周炎牙槽骨重建的影响 丁 一1，杨 恒1，文 钦2 (610041成都，四川大学华西口腔医院牙周科1；400015重庆，重庆医科大学附属口腔医院牙周粘膜科2) [摘要] 目的 研究补肾固齿丸对大鼠实验性牙周炎牙槽骨重建的影响，进一步探讨补肾固齿丸对牙周炎的治疗机制。方法 成功建立大鼠牙周炎模型之后，大鼠分为正常组、牙周炎模型组、补肾固齿丸组，给予补肾固齿丸治疗，采用放射免疫法测定血清骨钙蛋白与Ⅰ型胶原交联C端肽浓度，同时采用显微CT对大鼠牙槽骨进行三维重建，以评价补肾固齿丸对大鼠实验性牙周炎牙槽骨修复重建的影响。结果 经过补肾固齿丸治疗1个月后，治疗组补肾固齿丸组血清骨钙蛋白与Ⅰ型胶原交联C端肽浓度[分别为（0.859 1±0.156 9）、（2.038 6±0.407 5）]较之牙周炎模型组[分别为（1.806 4±0.258 5）、（3.056 0±0.461 5）]（1.8064±0.2585，3.0560±0.4615）分别下降为（0.8591±0.1569，2.0386±0.4075，（P0.05）。治疗组补肾固齿丸组大鼠牙槽骨的骨小梁体积分数（0.304 1±0.010 0）明显高于牙周炎模型组[（0.181 6±0.020 0），P0.05），]，且骨小梁数量（3.124 4±0.100 0）略高于牙周炎模型组[（2.088 9±0.200 0），P0.05]），而治疗组补肾固齿丸组的骨小梁分离度（0.575 1±0.020 0）较之牙周炎模型组（0.611 0±0.030 0），P0.05）有降低的趋势（P0.05）。 结论 补肾固齿丸能改善牙周炎大鼠牙槽骨代谢状况，促进牙槽骨改建，对牙周炎具有一定的辅助治疗作用。 [关键词] 补肾固齿丸；大鼠；实验性牙周炎；显微CT [中图法分类号] [文献标志码] A Effects of Bushenguchiwan on alveolar bone reconstruction of rats with experimental periodontitis Ding Yi1, Yang Heng1 , Wen Qin2 (1Deptartment of Periodontology, West China College of Stomatology, Sichuan University, Chengdu, 610041;2Department of Periodontology and Oral Medicine, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, 400015, China) [Abstract] Objective To explore the influence of Bushenguchiwan on alveolar bone reconstruction of rats with experimental periodontitis，and to discuss the treatment mechanism of Bushenguchiwan. Methods The model of experimental periodontitis of rats were established by means of steel wire, high- carbohydrate diet and Periopathogens noculation. We used Bushenguchiwan to treat them. The content variation of osteocalcin (OCN) and pyridinoline cross-linked carboxy-terminal telopeptide of type Ⅰ collagen (ICTP) in serum were observed by radioimmunoassay (RIA). Micro-CT and software digitized specimens were reconstructed three-dimensionally for parameter assessment of alveolar bone(bone volume /total volume , trabecular number , trabecular separation) .Results Rat experimental periodontitis mod