纳洛酮对弥漫性脑损伤合并二次脑损伤大鼠c.pdf

　 军 杂 2012年9月1 日　第37卷　第9期 694 解放 医学 志　 纳洛酮对弥漫性脑损伤合并二次脑损伤大鼠c-fos表达及β 内啡肽水平的影响 荆俊杰，王守森，杨庆武，王如密，高进喜，赵琳 [摘要]　目的　观察应用纳洛酮干预后，弥漫性脑损伤(DBI)合并二次脑损伤(SBI)大鼠脑内c-fos蛋白及血浆β内 啡肽( β-EP) 的表达变化，以进一步探讨β-EP与c-fos在SBI 中的作用。方法　健康雄性SD大鼠70只，随机分为DBI合并 SBI组(A组) 、DBI合并SBI后盐酸纳洛酮(1mg/kg ，腹腔注射)治疗组(B组) 、DBI后SBI前盐酸纳洛酮治疗组(C组) ，于伤 后3 、24 、48h处死大鼠，采用免疫组化及放射免疫分析法测定脑内c-fos蛋白和血浆β-EP 的含量，并进行脑组织病理 形态学观察。结果　B 、C两组在各时间点的各检测指标比较差异均无统计学意义(P 0.05) 。与B 、C两组比较，A组3h 及24h时的脑组织含水量明显升高(P 0.05) ，24h及48h 时的神经元损伤数量明显减少(P 0.05) ，3h 、24h及48h 时的c-fos 蛋白表达明显升高，3h及24h时的血浆β-EP含量也明显升高(P 0.05) 。结论　盐酸纳洛酮可降低DBI合并SBI大鼠脑内 c-fos蛋白及血浆β-EP 的含量，减轻神经损伤程度，具有一定的神经保护作用；DBI合并SBI后与DBI后SBI前使用盐酸 纳洛酮对SBI 的疗效相仿。 [关键词]　脑损伤；原癌基因蛋白质c-fos ；β内啡肽；纳洛酮 [中图分类号]　R651.15 [文献标志码]　A [文章编号]　0577-7402(2012)09-0694-04 Effect of naloxone hydrochloride on c-fos protein expression in brain and plasma beta-endorphin level in rats with diffuse brain injury and secondary brain insult 1 1 2 1 1 1 JING Ju n-jie , WANG Shou-sen , YANG Qing-wu , WANG Ru-mi , GAO Ji n-xi , ZHAO Lin 1Department of Neurosurgery, Fuzhou General Hospital of Nanjing Command, Fuzhou 350025, China 2Department of Neurosurgery, Second Hospital of Fuzhou, Fuzhou 350001, China [Abstract]　Objective　To observe the changes of c-fos protein expression in brain and beta-endorphin ( β-EP) level in blood plasma in rats with diffuse brain injury (DBI) and secondary brain insult (SBI) after intraperitoneal injection of naloxone hydrochloride, and explore the role of c-fos and β-EP in development of SBI in rats. Methods　Seventy health male SD rats were enrolled in the present study and randomly divided into group A (intraperitoneally injected with 0.9% saline after DBI and SBI model was reproduced), group B (injected intraperitoneally with 1.0mg/kg naloxone hydrochloride after DBI and SBI model was reproduced), and group C (intrape