米尔贝肟片在犬血浆中的药物代谢动力学研究-兽医学;基础兽医学专业毕业论文.docxVIP

Classified Classified Index： U．D．C： Dissertation for the Master Degree in Agriculture PHARMACOK玳ETIC STUDY OF MILB EMYCIN OXIME TABLET N DOG PLASMA Candidate： Yu Xiaolei Supervisor： Prof．Li Jichang Academic Degree Applied for： Master ofAgriculture Speciality： Basic Veterinary Medicine Date of 0ral Examination： June．201l University： Northeast Agricultural University 一 。量量 盐一．一．』蜊朔燃一。 -- 3．2米尔贝肟在犬体内的药物代谢动力学规律 23 3．2．3犬心安片口服给药药物代谢动力学 ．27 东北农业大学农学硕士学位论文4．1．2质谱条件的优化 东北农业大学农学硕士学位论文 4．1．2质谱条件的优化 33 4．1．3色谱条件的选择 34 4．1．4血浆样品的前处理方法 34 4．2米尔贝肟在犬血浆中的药物代谢动力学规律 35 5．结论 ．36 致谢 ．37 参考文献 。38 攻读学位期间发表的学术论文 。43 ContentsCoNTENTS Contents CoNTENTS Abstract in Chinese ．．I Abstract ．III 1．Intruduction ．1 1．1 Study on pharmacokinetics l 1．1．1 Pharmacokinetics and drug development ．1 1．1．2 Study on pharmaeokineties ofmacrocyclic lactones 。2 1．1．3 Application of LC／MS／MS in pharmacokinetics 8 1．2 Study on milbemycin oxime ．9 1．2．1 anysico—chemical property of milbemycin oxime 9 1．2．2 Action mechanism ofmilbemycin oxime 。10 1．2．3 Study on pharmacokinetics of milbemycin oxime ．11 1．2．4 Safety l l 1．3 Basis ofthe subject ． 12 2．Materials and methods 13 2．1 Materials ． ．13 2．1．1 Reagents ．1 3 2．1．2 Equipments 13 2．1．3 Animals 14 2．2 Methods 14 2．2．1 Pharmacokinetics ofmilbemycin oxime in dog blood ．14 2．2．2 Regularity ofmilbemycin oxime in dog vivo 。16 3．Results ． ．17 3．1 Establish ofthe detection method 17 3．1．1 Specificity ofthe mohod ．17 3．1．2 Standard curve and linear range ．．21 3．1．3 Limit ofquantification and detection 21 3．1．4 Coefficient ofrecovery and retrieval 21 3．1．5 Matrix effect ．22 3．1．6 Precision and accuracy 22 3．1．6 Stability test ． ．22 3．2 Regularity of milbemycin oxime in dog vivo 24 3．2．1 Physical examination of experiment dog 24 3．2．2 V．ein injection 24 3．2．3 Oral import milbemycin oxime ． ．．25 4．Discussion 31 4．1 Pharmacokinetics ofmilbemycin oxime in dogs ．31 4．1．1 Selection of detection method ．．3 1 4．1．3 4．1．3 Choice of chromatographic condition ．．32 4．1．4 Prc