马兜铃酸对肾脏管旁毛细血管内皮细胞的毒性作用-内科学(肾病)专业毕业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 万方数据 万方数据 缩略语表 英文缩写 AA 英文全称 Aristolochic acid 中文全称 马兜铃酸 AAN aristolochic acid nephropathy 马兜铃酸肾病 APS ammonium persulphate 过硫酸铵 ddH2O DMSO Double distillated water Dimethyl sulfoxide 双蒸水 二甲基亚砜 EDTA Ethylenedia minetetraascetic acid 乙二胺四乙酸 EMT Epithelial to mesenchymal transition 上皮细胞- 间充质转分化 FBS Fetal bovine serum 胎牛血清 HUVEC Human umbilical vein endothelial cell 人脐静脉内皮细胞株 HRP horseradish peroxidase 辣根过氧化物酶 LSCM Laser scanning confocal microscope 激光共聚焦扫描荧光显微镜 MTT Methylthio tetrazole 四甲基偶氮唑盐 OD Optical density 光密度 PBS Phosphate buffer solution 磷酸盐缓冲溶液 PMSF Phenylmethyl sulfonyl fluoride 苯甲基磺酰氟 PVDF Polyvinylidene difluoride 聚偏二氟乙烯 SDS Sodium dodecyl sulfate 十二烷基磺酸钠 TBST tris-buffered saline tween-20 Tris 盐-吐温缓冲液 TEMED Tetramethylethylenedia mine 四甲基二乙胺 Tris tris(hydroxymethyl) aminomethane 三(羟甲基)氨基甲烷 1 The toxicity effect of Aristolochic acid on peritubular capillary endothelial cells of kidney Abstract Background: Aristolochic acid (AA), belonged to Aristolochia, can cause kidney tubular necrosis, renal interstitial fibrosis and then result in chronic renal failure in patients who have AA or AA-containing drugs for a long-term. This type of kidney disease is generally defined as aristolochic acid nephropathy (Aristolochic Acid Nephropathy, AAN) by scholars throughout the world. The most of AAN patients showed renal tubular epithelial cell degeneration, necrosis, the lack of obvious epithelial cell regeneration, bare basement membrane, diffuse interstitial widening and progressive fibrosis, which are different from the other kidney diseases or toxic nephropathy. Currently, the mechanism of these special pathological injury is not very clear and there are not effective clinical treatments for AAN patients. So, AAN patients often delayed healing and eventually progress to end- stage renal failure. Therefore, it’s necessary to explore the tubular injury and repair mechanisms of AAN deeply. Scholars conducted a lot of researches about the tubule