酸浆宿萼中β.pdfVIP

158 2010, Vol. 31, No. 17 食品科学 ※基础研究 蔡 靳，赵 婷，惠伯棣 * ，宫 平 (北京联合大学应用文理学院，北京 100191) 目的：从酸浆(Physalis alkengi L. var. franchetii (Mast.) Makino)宿萼中制备 β- 隐黄质( β-cryptoxanthin)，应 用微弱发光测量方法对 β- 隐黄质体外淬灭单线态氧的能力进行评价。方法：用正己烷萃取酸浆宿萼中的β- 隐黄质 酯后经皂化获得单体，使用 C18-HPLC-PDA 对制备物进行定性和定量。用次氯酸钠 - 双氧水反应产生单线态氧，使 用微弱发光测量仪检测制备组分淬灭单线态氧的能力。结果：经 C18-HPLC-PDA 鉴定，制备物中的类胡萝卜素主 要为 β- 隐黄质。微弱发光测量仪的数据显示： β- 隐黄质的存在能减少次氯酸钠- 双氧水单线态氧体系中产生的最 大光子计数，且存在量效关系。0.049～0.49g/L 的 β- 隐黄质可减少50% 的最大光子计数。 宿萼； β- 隐黄质；单线态氧 in vitro Singlet Oxygen Quenching Ability Assessment of β-Cryptoxanthin from the Calyx of Physalis alkengi L. var.franchetii (Mast.) Makino CAI Jin ，ZHAO Ting ，HUI Bo-di* ，GONG Ping (College of Applied Arts and Science, Beijing Union University, Beijing 100191, China) Abstract ：Objective: To assess the ability of β-cryptoxanthin from the calyx of Physalisalkengi L. var. franchetii (Mast.) Makino to quench in vitro singlet oxygen. Methods: β-cryptoxanthin eater was extracted from the calyx of Physalisalkengi L. var.franchetii (Mast.) Makino with n-hexane and then saponified to produce β-cryptoxanthin fraction. The fraction was identified and quantified on C18-HPLC-PDA. Based on the reaction between NaClO and H2O2, singlet oxygen was produced. Singlet oxygen quenching ability of the β-cryptoxanthin fraction was determined on ultra-weak luminescience analyzer. Results: By C18-HPLC- PDA, the prepared fraction was identified as β-cryptoxanthin. The data from ultra-weak luminescience analyzer suggested that the β-cryptoxanthin fraction was able to reduce the maximum number of photon generated from the reaction solution in positive correlation with i