铝诱导不同耐铝型速生桉无性系的根尖细胞生物学响应机制分析-森林培育专业毕业论文.docxVIP

IV IV 细胞壁上的吸附位点增加，结果铝在植物根尖的累积量增加，同时细胞壁 PME 活性明显大，使已经大量分泌的果胶水解变成铝吸附性更强的果胶酸， 协同加剧了铝积累和毒害。尾叶桉 4 号细胞壁果胶、半纤维素Ⅰ半乳糖醛 酸与总糖比例降低，细胞壁活性降低，导致其韧性加强，根尖细胞伸长受 限制。而巨尾桉 9 号根尖细胞壁结构功能受铝毒干扰较尾叶桉 4 号的小， PME 酶活性变化不大。 （4）根尖铝在两个无性系的微区分布表现一致，亚细胞组分铝含量排 序为：细胞壁细胞浆细胞器，而细胞壁吸持的铝绝大多数都在果胶上。 铝处理后巨尾桉 9 号根尖、细胞壁、细胞壁果胶铝吸附量及细胞器和细胞 浆铝积累量均比尾叶桉 4 号少。镜检结果发现巨尾桉 9 号根尖表面损伤及 分生区细胞凋亡程度比较轻。 （5）变异系数综合评价结果表明巨尾桉 9 号耐铝机能比较高。 关键词：桉树 细胞壁 抗氧化酶 铝分布 细胞凋亡 PAGE PAGE VI CELL BIOLOGICAL MECHANISMS OF ALUMINUM INDUCED ON ROOT APEX OF DIFFERENT ALUMINUM-TOLERANT FAST-GROWING EUCALYPTUS CLONES ABSTRACT Two fast-growing eucalyptus clones E. grandis×E. urophylla GLug9 and E. urophylla GLu4 were chosen to study the effects of physiological and biochemical differences under aluminum in acidic environment induced for the research of different-resistant mechanism of Al effect on eucalyptus clones, high-quality strain breeding, improving the productivity of eucalyptus plantation in Al-rich red soil region and implementing sustainable management of eucalyptus plantation.Through analyzing root growth, root apex’s Al distribution, morphological structure changes of root apex cells and antioxidant physiology of roots and leaves of the rule of the fast-growing Eucalyptus clones under aluminum-induced, conclusions came to those as following: The root growth and activity of the fast-growing Eucalyptus clones were inhibited or decreased, while the cell membrane permeability of the roots and leaves increased, electrolyte dissolution and lipid membrane per oxidation. But there are differences in performance between clones, growth and activity of root suppressed much small, has limited quantities of electrolyte dissolution and lipid membrane per oxidation due to the less damage of cell membrane in G9 as strong aluminum tolerance clone than G4. Damage of leaves was slightly and hysteresis compared to the root under Al in two clones. The Antioxidant enzyme activity was improved in two clones under aluminum-induced. While activ