美金胺联合托吡酯对新生鼠缺氧缺血性脑损伤的神经保护作用分析-儿科学专业毕业论文.docxVIP

IV IV of the right hemisphere which was presented as the percentage of hemispheric weight loss of the right side relative to the left at 22d after HI. Data are presented as mean±S.D. Comparisons were performed by one-way ANOVA with Fisher’s post hoc test by using SPSS 15 for windows. Differences were considered significant when p0.05. Results Positive cell counting of TUNEL staining and caspase-3: In the CA1 sector of the hippocampus and subcortical white matter, the numbers of apoptotic cells in the combination group were significantly reduced compared with the vehicle group. The result of caspase-3 positive cell counting is consistent with that of TUNEL staining. Microscopic damage grading: The histopathologic score in the combination group were significantly lower compared with the vehicle group in the cortex, hippocampus and thalamus. Foot fault test: The number of foot-faults per pup was significantly less in the combination group than that in the vehicle group. Gross brain damage grading: The neurologic damage score was significantly lower in the combination-treated group than that in the vehicle-treated group. Wight deficit of the right hemisphere: The weight deficit of the right hemisphere in the combination group was significantly reduced compared with the vehicle group. Conclusion The combination of memantine and topiramate not only attenuates acute neural injury but also reduces brain injury in a long period. The combination of memantine and topiramate can reduce cellular apoptosis in the CA1 sector of the hippocampus and subcortical white matter. The combination of memantine and topiramate improved both pathological outcome and neurofunctional performance significantly. 【Key words】neuroprotection; memantine; topiramate; pharmacology; combination therapy; PAGE PAGE VII glutamate receptor; apoptosis 【缩略词及关键词表】 缩写 英文全称 中文全称 MEM Memantine 美金胺 TPM Topiramate 托吡酯 HI hypoxic-ischemic 缺氧缺血 HIE hypoxic-ischemic encephalopathy 缺氧缺血性脑病 HIBI hypoxic-ischemic bra