黄芩为唇形科植物黄芩Scutellariabaicalensis-中国新药杂志.DOCVIP

6,7-二乙酰黄芩素对CCl4和D-GalN所致的急性肝损伤的保护作用[ [基金项目] 国家自然科学基金 [作者简介] 刘晓岩，女，硕士，主要心血管药理学研究。联系电话：（010E-mail： liuyan@bjmu.edu.cn。 [通讯作者]王银叶，女，教授，主要从事心血管药理学研究。联系电话：（010E-mail： wangyinye@bjmu.edu.cn。 刘晓岩1，张超逸1，王银叶1，车庆明2 （北京大学药学院1分子与细胞药理系，2天然药物化学系，北京 100191） [摘要] 目的：研究6,7-二乙酰黄芩素对四氯化碳（CCl4）和D-氨基半乳糖（D-GalN）所致急性肝损伤的保护作用。方法：分别用CCl4和D-GalN诱导化学性急性肝损伤模型，测定血清中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）水平；并用苏木素-伊红（HE）染色处理肝脏组织切片，光镜观察病理学改变；用试剂盒测定肝线粒体中AST、SOD和GSH-PX的活性及脂质过氧化产物MDA含量。结果：在CCl4和D-GalN诱导和的小鼠肝急性损伤模型中，6,7-二乙酰黄芩素给药(50，100 mg?kg-1，ig)明显降低血清ALT、AST水平；明显改善肝脏病理组织状况；6,7-二乙酰黄芩素给药（25, 50, 100 mg?kg-1，ig）明显降低CCl4诱导的肝急性损伤小鼠的肝线粒体中AST活性和MDA的含量，显著增加SOD和GSH-PX的活性。结论：6,7-二乙酰黄芩对CCl4和D-GalN诱导和的小鼠肝急性损伤均具有保护作用，该作用与其增加线粒体中抗氧化酶的酶的活性、降低脂质过氧化水平有关。 [关键词] 6,7-二乙酰黄芩素；急性肝损伤；抗氧化；脂质过氧化 [中图分类号] R965.1；R975.5 [文献标识码] A [文章编号] Protective effects of baicalein 6,7-diacetate on CCl4 or GalN induced hepatic injury in mice LIU Xiao-Yan 1 ，ZHANG Chao-Yi1，WANG Yin-Ye1，CHE Qing-Ming2 （1 Department of Molecular and Cellular Pharmacology, 2 Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China） [Abstract] Objective: To study the protective effects of Baicalein 6,7-diacetate on acute liver injury induced by CCl4 or D-GalN in mice. Methods: The models of acute liver injury were induced by CCl4 or D-GalN in mice. The serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined, respectively. Liver tissue sections were examined under a light microscope. AST, SOD and GSH-PX activities and MDA content in liver mitochondria were also detected. Results: In the model of acute liver injury induced by CCl4 or D-GalN in mice, baicalein 6,7-dicaetate (50，100 mg?kg-1, ig) markedly decreased the elevated serum levels of ALT and AST. Baicalein 6,7-diacetate effectively improved the tissue lesion of liver. In addition Baicalein 6,7-diacetate (25, 50, 100 mg?kg-1, ig) significantly increased SOD and GSH-PX activities , an