氯磷酸盐脂质体对小鼠烫伤后瘢痕形成的影响和相关机制的研究-整形外科学专业毕业论文.docxVIP

汕头大 汕头大学医学院硕士学位论文 汕头 汕头大学医学院硕士学位论文 PAGE PAGE V V VI ABSTRACT Objective: The aim of the study is to learn about the effects of subcutaneous or intraperitoneal injection of clodronate liposomes on scar formation in a mouse model of burn injury and investigate the possible mechanism. Maerials and Methods: Sixty Kunming mice were randomly divided into the following 3 groups: the control group, the subcutaneous group and the intraperitoneal group. After the thermal injury was induced，mice in the control group were injected with saline. Mice in the subcutaneous group were subcutaneously injected with 50 μl of clodronate liposomes in the normal skin about 1cm beyond the thermal injury once immediately after the thermal injury was induced (day 0) and once on the next day (day 1). Mice in the intraperitoneal group were intraperitoneally injected with 200 μl of clodronate liposomes once immediately after the thermal injury was induced (day 0), once on day 2, and once on day 4. On day 5 after the injury, wound tissues were harvested for immunohistochemistry analysis to characterize the effect of clodronate liposomes on macrophage depletion. Wound healing time of each mouse was recorded during the experiment and scar tissues were harvested 2 weeks after epithelialization for hematoxylin and eosin (HE) staining to observe the scar histological characteristics, reverse transcription polymerase chain reaction (RT-PCR) and western blot analyses to characterize the effect of clodronate liposomes on TGF-β1 and collagen I-α2 expression in the scar. Results: Immunohistochemistry analysis showed an abundance of CD68+ macrophages in the wound tissue of the control group. In contract, the number of CD68+ macrophages in the intraperitoneal group was much less and no CD68+ macrophages were detected in the subcutaneous group. The healing time of the subcutaneous group and the intraperitoneal group was much longer than that of the control group (35.1±0.8 days, 28.6 ±1.0 days, and 21.4 ±0.7