洛伐他汀对LPC所致兔胸主动脉内皮功能损伤的保护作用-药理学专业毕业论文.docxVIP

洛伐他汀对kPO所致免胸主动脉 洛伐他汀对kPO所致免胸主动脉 内皮功能损伤的保护作用 中文摘要 目的：探讨洛伐他汀对溶血性磷脂酰胆碱(LPC)和二苯三硝基 苯肼(DPPH)所致家兔离体胸主动脉环内皮功能损伤的保护作用。 方法：用家兔离体胸主动脉环检测血管内皮依赖性舒张(EDR) 反应及血管组织的脂质过氧化代谢产物丙二醛(MDA)的含量和超氧 化物歧化酶(SOD)的活性。 结果：LPC(4mg／L)及DPPH(1 u mol／L)显著抑制了乙酰胆碱诱 导的内皮依赖性舒张反应，增加了血管组织中MDA的含量和降低了 SOD的活性。血管环预先与不同浓度(O．025、0．05、0．1 u mol／L)的 洛伐他汀孵育15min，再分别与LPC、DPPH共孵，可显著减轻LPC及 DPPH所致的EDR反应损伤，保护了血管组织中SOD的活性，减少了 MDA的含量。 结论：洛伐他汀对LPC和DPPH所致血管内皮功能损伤具有保护 作用。其作用机制可能与抗氧自由基损伤或增强NOS的活性，或促进 N0释放有关。 关键词：洛伐他汀，溶血性磷脂酰胆碱，二苯三硝基苯肼，内皮 依赖性舒张 Protective Protective effects of lovastatin Oil rabbit aortic endothelial dysfunction induced by LPC ABSTRACT AIM：To study the protective effects of lovastatin agaist vascular endothelial dysfunction induced by lysophosphatidylcholine(LPC)and 1．1-dipheyl一2-picryl hydrazyl(DPPH)． METHoDS：The endothelium dependent relaxation response (EDRRl to acetylcholine(ACh)was examined in the isolated rabbit thoracic aorta rings．Malondialdehyde fMDAl content and superoxide dismutase(SOD)activity in vascular tissue were measured． RESULTS： An incubation of aortic rings with LPC(4mg／L) medium for l 5mins or with DPPH fl I』mol／L)medium for 1 0 min，both LPC and DPPH significantly resulted in a decrease of EDRR induced bv ACh．decrease of SOD activity and elevation of MDA contents in vessel walls．An incubation of lovastatin at different concentration fO．025，0．05 and 0．1 u mol／L)for l 5 min prior to LPC or DPPH， significantly protected from inhibition of vascular EDRR and SOD activity and prevented from elevation of MDA content by induced bom LPC orDPPH． CoNCLUSION：Lovastatin Can significantly protect endothelial dysfunction induced by LPC and DPPH in vitro．The mechanisms may be related to decreasing oxidation induced by free radicals，preserving NOS activity,or increasing the release ofNO． KEY WORDS：lovastatin,lysophosphafidylcholine，l，1-dipheyl一2一picryl hydrazyl，endothefitwa-dependentrelaxation lI 原创性声明本人声明，所呈交的学位论文是本人在导师指导下进行的研究工 原创性声明 本人声明，所呈交的学位论文是本人在导师指导下进行的研究工 作及取得的研究成果。尽我所知，除了论文中特别加以标注和致谢的 地方外，论文中不包含其