黄连木香配伍对溃疡性结肠炎大鼠模型干预作用及相关机制分析-方剂学专业毕业论文.docxVIP

5 5 ABSTRACT Objective: This study aimed to change HuangLian and MuXiang compatibility with ulcerative colitis rat model of damp-heat in the intervention, while exploring rat colon SOD, MDA, Gin, TNF-a content of change and intervention of the HuangLian and MuXiang relevance. Methods: SD rats 90, male and female were randomly divided into 9 groups, divided into blank group, model group, positive medicine group, HuangLian group, MuXiang group, HuangLian and MuXiang 1:1 compatibility group, HuangLian and MuXiang compatibility group 2:1, 4:1 HuangLian and MuXiang compatibility group, HuangLian (Health) and MuXiang 1:1 compatibility group. In addition to the control group, the rest are used trinitrobenzene sulfuric acid (TNBS) / ethanol enema ulcerative colitis established. To HuangLian, the proportion of wood by pre-configured boiling water, adjust the drug concentration is 350mg/ml. Made film from the 1st day after the model group daily with saline, positive drug gentamicin sulfate injection group 28mg/kg/day treatment group 350mg/kg/day orally for 8 days. Observations: body weight, stool consistency and occult blood, DIA, the pathological changes of the colon, colonic SOD, MDA, Gin, TNF-a content. Results: ①model group were needy, lazy action, weight loss, then quality Qingxi, UC oid pus and blood will be part of the rats; model rats showed a different degree of colon injury, colon congestion observed the naked eye, erosion, multiple edema formation, and even intestinal necrosis; light microscope, colon luminal epithelial shedding, necrosis, inflammatory cells in the subm UC osa can be seen, suggesting that is UC PAGE PAGE 10 cessful model.② model group, the colonic tissue superoxide dismutase (SOD) content is lower than the control group, positive control group and treatment group than the model group, but lower than the control group, in which positive drug group, HuangLian and MuXiang 1:1 compatibility group, HuangLian and MuXiang 2:1compatibility group