十字花目GRFT分支的系统学研究-生态学专业毕业论文.docxVIP

Ab Abstract 山西 山西师范大学学位论文 PAGE PAGE III PAGE PAGE IV Title：The phylogeny study of GRFT clade of the Brassicales Major：Ecology Name：Zhao Huiling Signature： Supervisor：Yan Ming, Su Junxia Signature： Abstract GRFT clade was recognized by Hall et al. based on molecular evidence, which including Gyrostemonaceae, Resedaceae, Forchhammeria, and Tirania. The Molecular systematic study of Brassicales by Su et al. added two members to GRFT clade, Stixisand Borthwickia, and suggested Borthwickia as a new monotypic family. But the phylogenetic relationships and taxonomic status of the three members of GRFT clade, Forchammeria、Tirania and Stixis, are still unsolved. Therefore, Our study using the Maximum Parsimony and Bayesian analysis with ITS sequence reconstructed the phylogeny of the GRFT clade; Leaf epidermis of Borthwickia and Stixis were investigated using light microscopy (LM) and scanning electron microscopy (SEM); Twenty morphological characters of thirty-two taxa were selected for phylogenetic analyses. Using the Maximum Parsimony and Bayesian methods, we analysed the combined morphological and five DNA regions data for the GRFT clade, the main results are outlined as follows: Maximum Parsimony (MP) and Bayesian analysis (BI) of ITS sequences and five DNA regions strongly support GRFT clade as a monophyletic clade. Within the GRFT clade, Gyrostemonaceae is the earliest-diverging lineage, Borthwickia is resolved as sister to the rest clade, and other taxa formed a polyphyletic clade. The phylogenetic relationships within the GRFT clade is congruent with previous studies. Leaf epidermis of Borthwickia and Stixis were investigated using light microscopy (LM) and scanning electron microscopy (SEM). The results showed that: The characteristics of leaf epidermis of Stixisare very different from those of Capparaceae, Stixisis excluded from Capparaceae; The characteristics of