陆川猪与杜洛克猪正反交F1代肉质性状、肌内脂肪酶活性和相关基因的研究-动物遗传育种与繁殖专业论文.docxVIP

3、在肌肉组织学性状上，陆杜反交猪的肌纤维密度高于杜陆正交猪， 3、在肌肉组织学性状上，陆杜反交猪的肌纤维密度高于杜陆正交猪， 肌纤维直径低于正交猪。杂交F1代均位于两亲本间，但是陆杜反交猪更接 近于亲本陆川猪，说明肌纤维直径和密度倾向于父系遗传趋势。 4、在肌内脂肪相关酶活性的测定中，三组猪背最长肌中艘己、MDH 和ACC的酶活性均差异不显著。与肉质性状进行相关性分析发现，艘乙酶 活性与肌内脂肪呈显著负相关，MDH酶活性与肌内脂肪呈显著正相关，ACC 酶活性与肌内脂肪呈正相关，但相关性不显著。 5、与肌内脂肪相关基因的定量表达研究中，在三组猪背最长肌中FAS 基因的表达量与肌内脂肪相关性不显著；LPL和删基因表达量与肌内 脂肪呈正相关，但相关性均不显著；H-FABP基因表达量与肌内脂肪呈显著 正相关；Adiponectin基因表达量与背膘厚呈极显著负相关，其与肌内脂肪 呈负相关，但相关性不显著；PGC-1 a基因表达量与肌内脂肪无显著相关性。 关键词：陆川猪正反交F1代肉质性状肌纤维酶活性基因表达肌内脂 肪含量 万方数据 STUDIES STUDIES ON THE MEAT QUALITY TRAITS、 玳TR AⅫ，SCIⅡ，AR FAT ENZYME ACTⅣITIES AND RELATED GENESⅣUUCm，AN AND DURoC PIG RECPROCAL Fl In this article，the intercroSS F 1 generation of LuChuan and Duroc pig and their parents LuChuan pigs were analyzed of slaughter traits，muscle histology and meat quality,hybirds effects was compared between crossbred F 1 and their parents．QRT-PCR method was used to determine t mRNA expression levels of． intramuscular fat-related gene FAS,LPL，H-FABP,UCP3，Adiponectin，PGC-l a in LuChuan，DuLu and LuDu pig longissimus muscle．Enzyme activities and its changing law of LPL，MDH,A CC in longissimus muscles of three groups pigs were determined，and further research on the correlation between gene expressions，enzyme activities and IMF content．Discussed the pros and cons of heterosis and its molecular mechanism from better hybrids，has great significance for taking advantages of germplasm resources of local pig breeds in china，and could provide a scientific basis of developing new breeds from Chinese and foreign pig breeds． 1．Determination of slaughter,the LuChuan and Duroc pig reciprocal F I 万方数据 generation generation were better genetic good shape，hi曲lean meat percentage and bigger eye muscle area，make up the defects of small size and low lean meat percentage of LuChuan pigs．Slaughter rates and eye muscle areas of DuLu pigs were significantly higher than LuDu pigs，indicating that improvement effects of CarCasS traits in DuLu pigs is beuer than LuDu pigs． 2．In terms of meat quality,Meat