两个木薯品种组织培养再生体系建立的研究-植物学专业论文.docxVIP

II II Research on the Establishment of Regeneration System of Tissue Culture in Two Cassava Varieties Cen Xiangtao （College of Agriculture, Guangxi University, Nanning 530004,China） Abstract The research used the main cultivars of cassava SC124and SC205 as the experimental material for the study on the following aspects: it established the SC205 tissue culture’s rapid propagation system by taking SC205 stem with axillary buds as the explants and discussed the factors that influence the rapid propagation of cassava’s tissue cultures; It explored the factors that affect the regeneration of cassava by taking the lamina, stem and petiole of SC124 and SC205 as the experimental material, which aimed at providing theoretical and technical foundation for the rapid propagation of cassava’s isolated pieces and the genetic improvement through genetic engineering technology. The main research results are as follows: The third to sixth knob of cassava’s terminal bud is suitable for explants, this part is easier to sterilize, so the explants’ rate of survival is relatively higher; soaked the explants with 0.1% carbendazim solution for one-hour pretreatment can reduce the contamination rate; The appropriate sterilization method for the explants sampled on July to August is 75% ethyl alcohol for 20 seconds +0.1% HgCl2 for 10 min; The appropriate sterilization method for the explants sampled on September to October is 75% alcohol 20 s +0.1% HgCl2 for 12 min; The most appropriate sterilization method for hydroponic buds is process 5 min in 0.1% HgCl2 solution. The suitable primary culture medium for cassava’s sprouting of axillary bud is MS+6-BA0.5 mg/L, the suitable medium of subculture proliferation is MS+6-BA0.5 mg/L+KT0.5 mg/L and it proliferates in the form of bud, the growth coefficient reaches 4.5. The relatively suitable rooting medium is 1/2MS+NAA0.1 mg/L, the rooting rate is 100% and the average root number is 5.1. In the experiment of using two varieties of lamin