快速检测肠道致病菌的PCR技术的研究-食品科学专业论文.docxVIP

Rapid Rapid detection of enteropathogen with PCR assay Food seienee Candidate Dai juan Supervisor Li yufeng Abstract The problem of food safety is becoming more awaked and more serious for people in many counties．It is associated closely with the national development and people’S life,job aIld health．The mostly factor of food poisoning is bacterial food poisoning．The one of reasons causing human being disease is enteropathogen in food．The traditional detection of pathogeny microorganism need a long time，operation with trivial details．Meanwhile，the traditional detection Can’t get a perfectly identity result from some bacteria．There are more and more problem in our practice．So，we study the best advanced and sensitive PCR technology presently 抽develop a rapid,simple．precise quantity,sensitivity,speciality,economic detecting method．We study the familiar Enterobacteriaceae in our food to get a efficacious approach offood poisoning diagnose and medical treatment detection．In paper,there are Enterobacteriaeeae including Enterotoxigenic E．coli,Enteroinvasive E．coli,Shigella spp,falmonella spp． There are two or more pairs ofprimer in multiplex PCILIt Can amplify several DNA segments in a PCR．Multiplex PCR is effective，systemic and economic detecting method．The study applied multiplex PCR for detecting or identity a great deal of pathogeny microorganism．The special strain or genus gene sequence are III screened screened out．According to principle of primer designed，we progress to design primer．Then we amplify gene sequence of kinds of bacteria in a PCIL The multiplex PCR system is optimized by adjusting the concentration of MgCl2 and primer,the quantity of Taq 911zyme and template，the anneal temperature and cycle parameter．These primer of gene sequence Can amplify their DNA segment respectively．This multiplex PCR system is special and sensitive as well as the single PCR．The random combination is successful，We spend the same time amplifying in the multil：Ile