雷公藤内酯醇对皮肤鳞状细胞癌A431细胞株增殖与凋亡的影响-皮肤病与性病学专业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 摘要 目的研究雷公藤内酯醇(Triptolide，简称TP)对体外培养的人 皮肤鳞状细胞癌A43 1细胞株增殖与凋亡的影响。 方法1．MTT比色法测定TP对A431细胞株增殖活性的影响。 2．光镜观察细胞形态学的改变。 3．流式细胞仪检测TP作用于A431细胞株后细胞周期的分 布以及凋亡峰的出现。 结果1．随着TP剂量的增加及作用时间的延长，TP对A43l细胞 株增殖活性的抑制作用增强。 2．光镜显示TP能够引起细胞形态的改变。 3．TP能够改变细胞周期的分布，与对照组相比G0／G1期细 胞比例增多衅O．05)，S期细胞比例减少妒O．05)。 4．TP能够诱导A43 1细胞株凋亡。 结论TP呈明显剂量和时间依赖性抑制A431增殖，阻滞细胞周 期进程，诱导细胞凋亡。TP有可能作为临床上辅助治疗皮肤鳞状细 胞癌的药物之一。 关键词雷公藤内酯醇，鳞状细胞癌，A431，增殖，细胞周期，凋亡 硕士学位论文 硕士学位论文 英文摘要 ABSTRACT Objective： To investigate the effects of triptolide on the proliferation and apoptosis induction in epidermal squamous cell carcinoma cell line A43 l in vitro． Methods： Human epidermal squamous cell carcinoma cell line A43 1 was cultured．After the treatment with triptolide，inhibition of cellular growth was determined by measuring MTT dye absorption of the living cells． Light microscopy observed morphological changes．弧e DNA content and apoptosis were assessed by flow cytometry． Results： Triptolide could significantly inhibit the proliferation of A43 l cells with a dose—-and time·-dependent manner．Light microscopy revealed characteristic morphological changes of cell apoptosis．ne DNA flow cytometric analysis showed that triptolide could induce more sub—G 1 phase in A43 l cells，and cell cycle progression analysis which performed by flow cytometry indicated that cell cycle was obviously inhibited by triptolide in G0／G1 phase。 Conclusion： Triptolide plays an inhibitory role in the tumor cell proliferation and induces the cell apoptosis in epidermal squamous cell carcinoma cell line A43 1． KEY WORDS triptolide，carcinoma，squamous cell，A43 1， proliferation，cell cycle，apoptosis Il 硕士学位论文 硕士学位论文 缩略词表 缩略词表 卯 triptolide 雷公藤内酯醇 SCC squamous cell c毅cmoma 鳞状细胞癌 FCM flow cytometry 流式细胞术 DMEM Dulbeceo’S modified Eagle’S medium Dulbecco改良的Eagle培养基 FBS fet甜blood serum 胎牛血清 pMSO dimethyl sulfoxide 二甲基亚砜 嚣DTA ethylene diamin