丽蝇蛹集金小蜂卵黄原蛋白合成与摄取及卵黄磷蛋白降解的时间动态.PDF

浙江大李李报(农业与生命科学版)31(6):71卜718,2005 JournalofZhejiangUniversity(Agric.LifeSe.) 文章编 号:1008-9209(2005)06-0714-05 丽蝇蛹集金小蜂卵黄原蛋白合成与摄取及 卵黄磷蛋白降解的时间动态 姚鹏程，叶恭银，胡革 (浙江大学 应用昆虫研究所，浙江 杭州 310029) 摘 要:采用ELISA测定法检测了蝇类蛹期外寄生蜂 丽绳蛹集金小蜂印黄原蛋白合成与摄取，及卵 黄磷蛋白降解的时间动态 结果表明:该蜂卵黄原蛋白的合成与摄取分别起始于寄生后的第 10d和第 13d,即正处于该蜂的蛹初期和隐成虫期;胚胎内的卵黄磷蛋白随其发育过程而降解，寄生后第 3d,即 胚胎发育结束时则全部降解完毕 此外 还探讨了卵黄蛋白合成摄取和降解过程中，血淋巴.印桨或卵 内可浓性蛋 白含量 的的时间动态 变化 . 丽蝇蛹集金小蜂;卵黄原蛋白;卵黄磷蛋白;可溶性蛋白;ELISA 0492 文献标识码 :A YAOPeng-cheng,YEGong-yin,HUCui(InstituteofAppliedEntomology,ZhejiangUniversity, 月angzhou310029,China) TemporalpatternsofvitellogeninsynthesisanduptakeaswellasvitellindegradationinNasoniavitripennis (Hymenoptera:Pteromalidae).JournalofZhejiangUniversity (Agric. LifeSci.)，2005,31(6): 714-718 Abstract:Temporalpatternsofvitellogenin (Vg)synthesisanduptakeaswellasvitellin (Vn) degradationinNasoniavitripennis(Hymenoptera:Pteromalidae),anectoparasiticwaspofflypupae, werestudiedusingthemethodofenzyme-linkedimmunosorbentassay(ELISA).Theresultswere showedasfollows:thesynthesisanduptakeofVgofthisparasiticwasprespectivelyinitiatedsincethe lothand13thdayafterparasitism,whichwereequivalenttothefirstpupalstageandthepharateadult stageofthisparasiticwasp.ThedegradationofVnineggsofthisparasiticwaspwassimultaneouswith theirembryodevelopmentintheirhosts.TheVnthoroughlydegradedonthe3rddayafterparasitism, justbeingattheendofembryodevelopmentofthisparasiticwasp.Inaddition,thetemporalpatternof changeinlevelsofsolubleproteinsinhemolymph,ovariesoreggsofthisparasiticwaspwasalsodefined duringtheprocessesofVgsynthesisanduptakeaswellasVndegradation. Keywords:Nasoniavitripennis;vitellogenin,vitellin;solubleproteins;ELISA 昆虫卵黄发生(Vitellogenesis)是指卵黄原 蛋白(Vitellin,Vn)的过程.该过程及其内分泌调 蛋白(Vitellogen