山地杨的组织培养和快速繁殖的研究-园林植物与观赏园艺专业论文.docxVIP

摘 摘 要 本文以日本王子造纸公司培育的美洲黑杨(Popu]us de]toides，简称DD)与辽 杨(户maximowiczii，简称MM)的杂交品种(尸maximowiczii×尸de]toides,简 称MD系列)MDll0品系的两年生穗条为材料，以水培萌条的顶芽、叶片、叶柄为外 植体，探索了各外植体的消毒方法，愈伤组织诱导及分化芽与根的最佳配方，系统 研究了山地杨快速繁殖的过程及其影响因素。 以在室内水培萌发的芽、叶片、叶柄为外植体进行消毒处理。结果表明：用芽 作外植体时，先用70％酒精消毒lOsec，再用0．I％HgCI，加吐温20(数滴)消毒12min， 效果最好；叶片、叶柄用70％酒精消毒lOsec，再用0．I％HgCI：加吐温20(数滴)消毒 10min，效果最好。 在进行愈伤组织的诱导培养时，以芽的生长点为外植体诱导愈伤组织的效果最 好。MS+I．15mg／l 6-BA+2．5％蔗糖为最佳诱导培养基，诱导率为100％。 在愈伤组织增殖培养中，在细胞分裂素6-BA含量比较低的培养基上，生长较快， 30d直径最大能够增殖到1．1cm，愈伤组织的增殖率为100％，且以后容易诱导出小 苗。从经济观点和增殖效率上讲，最适合愈伤组织增殖的培养基为MS+I，15 mg／1 6-BA+2．5％蔗糖。 在愈伤组织的芽分化培养中，在固体和液体培养基上均能使愈伤组织分化出不定 芽。在琼脂固体培养基上，1，2叫)MS+0．3mg／16一BA+NAAO．1mg／l+2．5％蔗糖为最佳分 化培养基组合，其分化率可达94％。在MS+0．2mg／16一BA+2．5％蔗糖的液体培养基上， 芽的分化率也可以达98％。 在生根培养中，与萘乙酸相比，吲哚丁酸更有利于生根，且根生长粗壮。生根效 果较好的培养基为1／2(N)MS+0．05mg／1 NAA+2．5％蔗糖。在试管苗移栽中，以蛭 石为基质的移栽苗成活率最高，达到90％。以园土与蛭石为基质，其透气性、保湿 性虽然比单独的蛭石差，但生长比在其他基质中健壮，且在进入苗圃前不用再次移 苗。 关键词：山地杨组织培养愈伤组织快速繁殖 Studiiss Studiiss on ti ssue cuIture and rapid propagation of the GUItivar MDl 10 of Populus maximowicziix P de／toides Wang jianya (Col lege of Horticulture and Forestry Science，Huazhong Agricultural University Wuhan，430070) Abstract In this paper,the sterilizing procedures were studied by using different explants including buds，leaves and petioles from the two—year—old cutting shoots of MD I 00 of Populus maximowiczii×户deltoides from oji Paper Co．．Ltd．The callr5 was induced from different explants，the buds and roots were differentiated from the callus． The sterilizing methods of the buds，leaves and petioles sprouted from the cutting shoots cultured in water in a greenhouse were investigated．The results showed that the optimal sterilizing method of buds was dipping into 70％alcohol for 1 0 sec，and then treating with 0．1％HgCl2 plus Tween一20(4 drops)for 12 rain．The contamination of the leaves and petioles in culture was largely improved when they were treated with 70％ alcohol for 10 SCC，and then treated、Ⅳith O．1％HgCl2 plus Tween一20(4 drops)for 10 min． The effect of medium and hormone