森登4汤乙酸乙酯提取物成分的分离鉴定及其TLC质控方法研究-有机化学专业论文.docxVIP

摘 摘 要 本研究根据森登一4汤不同溶剂提取物抗风湿药效比较研究实验结果，对蒙药森 登一4汤水煎剂的乙酸乙酯萃取物进行提取、分离、纯化及结构测试，从中鉴定出两 个化合物。它们分别为2c【，3p．双氢杨梅树皮素没食子酸酯和2C【，3p一双氢杨梅树皮素。 建立森登一4汤中的槲皮素、没食子酸、阿魏酸定性分析的薄层色谱(TLC)方法， 并对药物中的槲皮素含量进行了测定。本实验对蒙药森登一4汤的二次开发研究以及 制剂质量控制提供实验依据。 关键词：森登．4汤；化学成分；质量控制；TLC Study Study on Separation and Identification of Constituents in Ethyl Acetate Extraction of Sendeng一4 Decoction and TLC Method Abst ract Based on the previous comparative study on the anti—inflammatory and analgesic effects of different extraction of Sendeng．4 decoction，Ethyl Acetate was selected as the extraction solvent．Through the separation，purification and identification of the Ethyl Acetam extraction of sendeng-4 decoction，two compounds such as 2et，3 p-dihydromyricetin and 2Q，3p．Dihydromyricetin gallate were isolatated and identified．The qualitative analytical method，Thin Layer Chromatography(TLC)method for identification of Quercetin，Gallic acid arid Ferulic acid in Sendeng-4 Decoction was established，and TLC quantification of Quercetin in the preparation was carried out．The present study provide the experimental basis for not only further development of Mongolian medicine Sendeng-4 decoction，but also quality control ofthe preparation． Key words：Sendeng-4 Decoction：Chemical constituents i Quali“．．Control Method；TLC Di rected by：prof．XU kiang AppI icant for Master degree：GAO Yang tOrganic chemistry．) (coliege of Chemistry，Inner Mongolia University For The Nationalities．Tongliao 028000．China) 插图和附表清单1．表1 插图和附表清单 1．表1 致小鼠耳肿胀作用的比较 ． ．．9 2．表2致小鼠扭体反应效果的比较 ． ．．10 3．表3薄层色谱展开条件 12 4．表4样品含量测定 ．15 5．图1 森登一4汤正丁醇提取物抗风湿活性成分提取分离流程图 7 6．图2 2 a，3 8一双氢杨梅树皮素没食子酸酯结构 ．12 7．图3 2 Q，3 13一双氢杨梅树皮素结构 ．．12 8．图4样品展开图 13 9．图5槲皮素样品及对照品展开图 ．14 lO．附图1 2Q，3p．双氢杨梅树皮素(2a，313-dihydromyricetin)H谱图 ．16 11．附图2 2Q，3p一舣氢杨梅树皮素(2a，3[Mihydromyricetin)C谱图 ．1