老年人血清对人脐静脉内皮细胞功能影响-老年医学专业论文.docxVIP

Abst Abstract PAGE PAGE IV ABSTRACT Objective： This study used human umbilical vein endothelial cells (HUVECs) as experimental materials,and used serum of the healthy elderly and gerontal patients with hypertension as damage factors,we established a injured model of HUVECs that reflect the characteristics of healthy elderly and patients with hypertension. HUVECs was cultured in each serum group,we utilized MTT method to detect the growth of HUVECs,and investigated effects of serum on endothelial cells in aging by testing changes in Nitric Oxide(NO) 、 Endothelin-1(ET-1) and Reactive Oxygen Species (ROS). Methods: The HUVECs was extracted from newborn umbilical cord which is taken from obstetrics in first affiliated hospital of Nanchang University,then we identified endothelial cells and culturing it 3-5 generations to be standby.We collected 20 serum samples from healthy young,the healthy elderly and gerontal patients with hypertension. The experiment was divided into 4 groups:blank control group (only add culture medium without serum),healthy young group,the healthy elderly group and gerontal hypertension group.We cultured HUVECs in each serum group,and then collected cell supernatants and cell at different times.we utilized MTT method to research the growth of HUVECs,redox method for detecting NO,ELISA method for detecting ET-1,Flow cytometry for detecting intracellular ROS.At last,we uesd statistical methods to process this experimental data,study the differences in each group. Results: The cell proliferation of each treatment group at the different time point has no significant difference on statistics (P0.05),and at the same time point,the cell proliferation of healthy young group,the healthy elderly group and gerontal hypertension group has no significant difference on statistics (P0.05),but blank control group has different with other groups (P0.05); The contents of NO in healthy young group,the healthy elderly group and gerontal hypertension group