快速分析彝药桃树寄生抗氧化成分-食品科学.PDFVIP

16 2012, Vol. 33, No. 01 食品科学 ※基础研究 1 1 2 1 朴香兰 ，邓长芹 ，陈虎彪 ，王 进 (1. 中央民族大学中国少数民族传统医学研究院，北京 100081 ；2. 香港浸会大学中医药学院，香港) 目的：快速分离、鉴定彝药桃树寄生的抗氧化成分。方法：通过1,1- 二苯基 -2- 三硝基苯肼(DPPH) 自 由基清除实验，评价彝药桃树寄生的甲醇提取物及其二氯甲烷、正丁醇及水萃取物的抗氧化作用，利用生物活性 - 高效液相跟踪方法，快速分离、鉴定其有效成分。结果：从彝药桃树寄生的二氯甲烷萃取物中分离、鉴定出有 效成分为松脂酚(pinoresinol) 。结论：利用生物活性- 高效液相跟踪方法可以快速分离、鉴定彝药桃树寄生中的抗 氧化成分，可应用于其他天然植物活性成分的快速筛选。 彝药桃树寄生；DPPH 自由基清除作用；生物活性- 高效液相跟踪方法；松脂酚 Rapid Analysis of Antioxidant Constituents from Taxillus delavayi, a Yi Ethnomedicinal Material 1 1 2 1 PIAO Xiang-lan ， DENG Zhang-qin ，CHEN Hu-biao ，WANG Jin (1. Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China ； 2. School of Chinese Medicine, Hong Kong Baptist University, Hongkong, China) Abstract ：Objectives: To rapidly separate and identify antioxidant constituents from the whole plant of Taxillus delavayi, a Yi ethnomedicinal material, and assess their antioxidant activity. Methods: The antioxidant activity of the methanol extract of the medicinal material and its different solvent fractions obtained by repeated extractions sequentially with dichloromethane, n-butanol and water was assessed by DPPH free radical scavenging assay. The dichloromethane-soluble fraction was further separated by silica gel column chromatography, and a high antioxidant component named as TDD8 was obtained and analyzed by bioassay-linked HPLC and 1H-NMR. Results: An individual compound named as TDD8-2 was separated from TDD8 by semi- preparative HPLC and ident