应用DGGE技术分析流清河湾扇贝养殖海区细菌群落结构的季节变化.doc

PAGE PAGE 1 表1 DGGE分离的部分获序列在GenBank中的Blast搜索 Tab.1 Blast of excised DGGE band in GenBank 条带 band 序列号Genbank accession no. 碱基数/bp bases number 亲缘关系最近的序列 most related sequence 相似度/% sequence similarity 归属类群taxonomic group 条带3 HQ161881 547 Uncultured Bacteroidetes bacterium clone A13W_189 (HM057642) 99 Bacteroidetes 条带5 HQ161882 547 Uncultured Flavobacterium sp. clone ARTE1_247 (GU230408) 99 Bacteroidetes 条带6 HQ161883 522 Uncultured alpha proteobacterium clone C13W_53 (HM057678) 99 α-Proteobacteria 条带8 HQ161884 528 Uncultured actinobacterium clone A13W_132 (HM057631) 99 actinobacteria 条带10 HQ161885 518 Uncultured actinomycete OCS155 (AF001652) 99 actinobacteria 条带11 HQ161886 523 Roseobacter sp. WED1.1 (AY536562) 99 α-Proteobacteria 条带12 HQ161887 541 Uncultured Bacteroidetes bacterium gene (AB266020) 99 Bacteroidetes 条带14 HQ161888 523 Rhodobacteraceae bacterium IMCC1933 (GQ468664) 99 α-Proteobacteria 条带16 HQ161889 505 Uncultured actinobacterium clone CB01B02 (EF471560) 99 actinobacteria 条带17 HQ161890 511 Rhodobacteraceae bacterium RCA23 (GQ468661) 99 α-Proteobacteria 条带18 HQ161891 500 Uncultured alpha proteobacterium clone CB01D03 (EF471712) 99 α-Proteobacteria 条带19 HQ161892 541 Uncultured Bacteroidetes bacterium clone SHBC1107 (GQ350184) 100 Bacteroidetes 条带22 HQ161893 545 Uncultured Verrucomicrobia bacterium clone SGSO653 (GQ347889) 99 Verrucomicrobia 条带23 HQ161894 499 Uncultured Roseobacter sp. clone 2_C6 (EU600651) 98 α-Proteobacteria 条带24 HQ161895 493 Uncultured Roseobacter sp. clone BL03-SUM34 (DQ778199) 99 α-Proteobacteria 条带25 HQ161896 530 Uncultured beta proteobacterium clone PI_4r8c (AY580385) 99 β-Proteobacteria 条带27 HQ161897 527 Limnobacter sp. Aph2 (FJ377401) 99 β-Proteobacteria 条带28 HQ161898 549 Uncultured gamma proteobacterium clone SGPW537 (GQ346769) 100 γ-Proteobacteria 条带30 HQ161899 527 Uncultured Sphingobacteriales cum Crenotrichaceae bacterium clone (FJ745018) 99 Bacteroidetes 条带31 HQ161900 518 Uncultured gamma pr