临床色谱质谱检验技术第三章 生物样品制备三.ppt

Pros and cons of sample Prep tech. Dilution- no cleanup or enrichment, good, simple, fast, clean high - Centrifugation- good clean up, separates by mass, no enrichment, cumbersome and expensive Filtration- no enrichment, good simple fast clean high detection limits Liquid/Liquid Extraction, Best clean up (multi step), separates by chemical properties, enrichment, cumbersome automate and expensive Solid Phase Extraction-good clean-up, fast , easy to automate, most options-(vendors and products) 这种使蛋白质分子凝聚并从溶液中沉淀析出的样品制备技术被称为蛋白沉淀法。 * 样品净化程度低， 仅可去除约90-95%的蛋白质，而基质中的高盐份和其他内源性干扰物如磷脂等完全未被去除； 分析柱寿命较短