纤维素酶产生菌的筛选及其相互作用研究-河南农业科学.pdf

河南农业科学 纤维素酶产生菌 筛选及其相互作用研究 1 1 1 2 卢月霞,尹会兰 ,黄慧敏 ,杨海如 (1.河北工程大学 农学院, 河北 邯郸 056021;2.井陉县农牧局, 河北 井陉051300) 摘要:从森林落叶土、腐烂 秸秆和农家堆肥等含有木质纤维素降解菌 样品中分离到能较好降解 纤维素 菌株4 株, 初步判定为3 株细菌, 1株放线菌。各菌株产酶(CMCase)较适宜 温度均是 37 ℃,培养基初始pH 值7.0,接种量(V/V )2%,接种培养12h 种子液酶活较高。对各菌株单独与 混合培养研究表明:菌株组合D6/D7、D1/D7、D6/D7/B7和D1/D6/D7/B7 在72h 酶活均显著高于 其单一菌株,其中最优组合D6/D7在72h 酶活可达67.12U/mL,相当于其菌株单独培养酶活 2倍。 关键词:纤维素降解;混合培养;纤维素酶;协同作用 中图分类号:Q939.96　　文献标识码: 　　文章编号:1004-3268(2010)01-0059-04 Screening of CMCase Producing Microorganisms and Their Interaction 1 1 1 2 LU Yue-xia ,YIN Hui-lan ,H U NG Hui-min ,Y NG Hai-ru (1.Department of griculture, Hebei University of Engineering, Handan 056021, China; 2.griculture Herding Bureau of Jingxing County, Jingxing 051300, China ) Abstract:Four strains of microbes with higher cellulase activity were selected from the samples of forest defoliation soil, rotten stalk and farmhouse compost at primary examination.Three were known as bac- teria, one was known asA ctinomyces .Their optimum enzyme-producing conditions were 37 ℃, medium primary pH at 7.0, and 2%of inoculation, inoculum age at 12h.These strains of microbes were also cultured solely and mixedly.The results showed that CMC enzyme activity at 72h of D6/D7, D1/D7, D6/D7/B7 and D1/D6/D7/B7 were obviously higher than that of the single strain, in which D6/D7 was the best,its enzyme activity reached 67.12U/mL, equal to double of each single strain.This study pro- vided theoretical basis for combination of superior strains and their co-operation in gene engineering. Key words:Cellulose degradation;Mixed Culture;Cellulase activity;Co-operation 　　纤维素是地球上最