丹参与三七配伍对主要活性成分药代动力学行为的影响.doc

· PAGE 1436· 药学学报 Acta Pharmaceutica Sinica 2010, 45 (11): 1433?1439 张丝韵等: 丹参与三七配伍对主要活性成分药代动力学行为的影响 · PAGE 1437· 药学学报 Acta Pharmaceutica Sinica 2010, 45 (11): 1433?1439 · PAGE 1433· 丹参与三七配伍对主要活性成分药代动力学行为的影响 张丝韵, 宋 敏*, 卢俊钢, 杭太俊 (中国药科大学药物质量与安全预警教育部重点实验室, 江苏 南京 210009) 摘要: 比格犬分别灌胃给药丹参、三七以及丹参-三七配伍的混悬液, 用LC-MS/MS法测定犬血浆中丹参素、丹参酮ⅡA、隐丹参酮、三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的浓度, 应用BAPP2.0计算药代动力学参数并进行统计分析, 通过比较药代动力学参数在配伍前后的变化探讨丹参与三七的配伍对这6个成分药代动力学行为的影响。结果显示, 配伍后丹参素、三七皂苷R1、人参皂苷Rg1和Rb1的Cmax均减少且AUC明显降低、CLz/F和Vz/F均有一定程度的增加, 而丹参酮ⅡA、隐丹参酮的药代动力学行为未见明显变化。 关键词: 丹参; 三七; 方剂配伍; 药代动力学; LC-MS/MS 中图分类号: R969 文献标识码: A 文章编号: 0513-4870 (2010) 11-1433-07 Effects of combination of Salvia miltiorrhiza and Panax notoginseng on thepharmacokinetics of their major bioactive components in Beagle dog ZHANG Si-yun, SONG Min*, LU Jun-gang, HANG Tai-jun (Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Nanjing 210009, China) Abstract: After oral administration of Salvia miltiorrhiza (Danshen in Chinese), Panax notoginseng (Sanqi in Chinese) and Danshen Sanqi combination suspensions to Beagle dogs, the plasma concentration-time profiles of danshensu, tanshinone ⅡA, cryptotanshinone, notoginsenoside R1, ginsenoside Rg1 and Rb1 were analyzed by LC-MS/MS. Pharmacokinetic parameters were calculated and analyzed with BAPP 2.0 software. The results showed that the Cmax and AUC of danshensu, notoginsenoside R1, ginsenoside Rg1 and Rb1 in Danshen Sanqi combination group all decreased in comparison with those of Danshen or Sanqi given alone, while the CLz/F and Vz/F increased to some extent. No significant differences of the pharmacokinetics of tanshinone ⅡA and cryptotanshinone were observed between groups. Key words: Salvia miltiorrhiza; Panax notoginseng; combination formulation; pharmacokinetics; LC-MS/MS 收稿日期: 2010-04-25.基金项目: 国家教育部博士点新教师基金资助项目 (2009009