耐盐耐热型纤维二糖酶的分离纯化和性质研究.pdf

26 2 No.2 Vol.26 2012 4 Journal of Chemical Engineering of Chinese Universities Apr . 2012 1003-9015(2012)02-0259-06  , , ( , 310027) Aspergillus sp. 10.98 Umg1 131.21 Umg165 pH 4.5pH NaCl 40 gL1 20~150 gL1 NaCl NaCl 2~3 pH TM344.1 Q556 Q814.1 A Purification and Characterization of a Salt-Tolerant and Thermostable Cellobiase HE Ke-ke, XUE Dong-sheng, YAO Shan-jing (Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China) Abstract: The Aspergills sp. isolated from the marine sediment was used to produce cellobiase via solid state fermentation. The cellobiase was obtained by ammonium sulfate precipitation, ultra-filtration and anion exchange chromatograph. The specific activity of the purified cellobiase increases to 131.21 Umg1 from that of the crude cellobiase (10.98 Umg1). It was found that the optimal temperature and pH for the activity of the cellobiase are 65 and 4.5, respectively, and in the solution with NaCl concentration in the range of 20~150 gL1, the activity of the cellobiase is higher than that in the NaCl- free solution; so it is a typical salt-tolerant enzyme. Moreover, the thermostability of the purified cellobiase is remarkably improved in high salinity solution, and its half-life time increases by 2~3 folds of that in salt-free solution. It was also found that both the activity and thermostability of the cellobiase produced from the marine Aspergills sp. are better than those from other terrestrial fungus. The purified cellobiase is thermo-stable and salt-tolerant and shows great pote