绿萝离体快繁与影响叶色变异因素的研究-园艺专业论文.docxVIP

AbstractThis Abstract This test mainly selected the explant and the steriling time，and researched the influence of different growth regulator callus induction，adventitious bud regeneration，multiplication of subculture，control of aerial root，and induced of adventitious root by tissue culturing of Epipremnum aureum and Scindapsus vat．wilcoxii．And this test solved the problem of browning at the callus induction and subculture process，finally it established the completed tissue culture and rapid propagation system of Epipremnum aureum．Moreover,this test has studied the variation of the color leaf in the tissue culturing，which included the major factors for the variation，the separation of this character and expanded propagation the pure variety．The primary test results follows： 1 At the primary culture of Epipremnum auyeum，the best explant is stem，and the optimal way of sterilization is secondary sterilization for 8+1 0min．The best medium for callus induction is MS+TDZ2．0mg／L+2，4．D0．2mg几．At the multiplication of subculture process，first，the best medium for adventitious bud regeneration is MS+6一BAl．0mg／L+NAA0．3mg几+TDZ0．2mg／t,．Second，the best enrichment culture medium is MS+6一BAl．Omg／L+NAAO．2mg／L，and injecting the PP333 about 0．2mg／L，which growth coefficient be enhanced obviously．Moreover，inj ecting 1 5ml perlite per 50ml solid medium control the growing of aerial root better．The best medium for the abduction of adventitious root is 1／2MS+NAA0．2mg／L． 2 The rate of browning of Epipremnum aureum is higher than Scindapsus wilcoxii，and the rate of browning of stem is higher than stipe and leaf．In the callus induction section,joining 2．09／L activated carbon 8．09／L citric acid and cultivating in darkness condition control the browning better．In the subculture and enrichment culture section，the rate of browning is more and more higher with the times of subculture increased．Injecting 1．Og／L activated carbon before the fourth subculture， 2．09／c activated carbon before the eighth s