灵芝属其中赤芝Ganodermalucidum-屏东科技大学食品科学系.docVIP

國立屏東科技大學食品科學系碩士班 研究生專題報告書面摘 題目：探討靈芝子實體多醣及菌絲體液態培養之胞外多醣體結構特徵 指導教授：郭嘉信 博士 及刺激免疫調節之影響 報告者 ：王韞皓 Comparisons of Immunomodulatory Effects and Structural Features 報告日期：2008/5/9 of Polysaccharides Fractionated from Fruiting Body and Submerged Culture of Ganoderma lucidum 摘要 靈芝(Ganoderma lucidum)萃取物具有調節免疫反應，增加人體的先天性及後天性免疫功能。靈芝萃取物之主要成分為β-多醣體（β-polysaccharides）、三帖類（triterpenes）、蛋白質、核酸、固醇（steroids）等，而其中最受矚目且研究生理活性最多的應屬高分子多醣體，高分子多醣體會與免疫細胞作用而啟動免疫細胞內的訊息傳遞，進而活化免疫細胞，增強淋巴細胞的增生能力及加強巨噬細胞的吞噬能力，以達到增進人體免疫功能之效果。靈芝菌絲體培養液經酒精沉澱之多醣體與靈芝子實體經30℃或80℃水萃獲得之多醣體經FPLC搭配S300管柱分離純化後，獲得不同分子量多醣體之液相層析指紋圖譜及分液樣品（fractionated samples），實驗規畫，取100μg不同來源的多醣體，加到106個週邊血液淋巴細胞中，進行培養72小時後，再利用MTT及流式細胞儀檢測其增殖效果。結果發現靈芝菌絲體培養液經酒精沉澱之多醣體可誘導T細胞增殖達控制組的3.45倍；而靈芝子實體經30℃或80℃水萃之多醣體可分別誘導T細胞增殖達控制組的5.42倍及4.92倍；相同地，靈芝菌絲體培養液經酒精沉澱之多醣體可誘導B細胞增殖達控制組的3.75倍；靈芝子實體經30℃或80℃水萃之多醣體可分別誘導B細胞增殖達控制組的5.28倍及5倍；結果顯示靈芝菌絲體培養液經酒精沉澱之多醣體與靈芝子實體經30℃或80℃水萃獲得之多醣體有增強免疫活性的效果。目前正進行具免疫活性的分液樣品，並檢測分液樣品中單醣組成及其多醣體結構，本研究期待了解靈芝菌絲體培養液經酒精沉澱之多醣體與靈芝子實體經30℃或80℃ 關鍵字：靈芝，多醣體，免疫調節 ABSTRACT Extracts of Ganoderma lucidum are considered to be capable of regulating immunomodulation and boosting innate and acquired immune systems in human body. The major components of the Ganoderma lucidum extracts are β-polysaccharides, triterpenes, proteins, nucleic acids, steroids, etc. Among those compounds, β-polysaccharides are the one which attract the most attention as well as research the most often on their physiological functions. β-Polysaccharides can interact with immunocytes and trigger their signal transductions, which may then activate the immunocytes, increase the multiplication of lymphocytes, and enhance the phagocytosis of macrophages, to boost up the functions of the human bodys immune system. we are using a FPLC equipped with a S300 column to fractionate the polysaccharides of Ganoderma lucidum from different sources to obtain polysaccharides fingerprintings and their fractionated samples containing polysacch