伸筋活血合剂TLC鉴别_杨婉花.pdfVIP

T LC Δ 1 * 1 2 杨婉花 , 陈 冰 ,徐佳妮 (1.上海交通大学医学院附属瑞金医院药剂科, 上海市 200025 ;2 .上海中医药大 学,上海市 200032 ) R283.665;R927.1 A 100 1-0408 (2009 )15-1164-03 目的:建立伸筋活血合剂的定性鉴别方法。方法:采用薄层色谱(TLC )法分别鉴别该制剂中伸筋草、白芍、续断 甘草。结 果:供试品中白芍、续断 甘草的色谱分别在 对照品色谱相对应的位置上显相同颜色的斑点, 阴性对照无干扰;但供试品中伸筋 草的色谱无特征斑点。结论:所建立的白芍、续断、甘草的TLC 鉴别方法重现性好、专属性强、结果可靠,可用于伸筋活血合剂的质 量控制。鉴于伸筋草的T LC 无特征斑点,建议改进伸筋活血合剂的生产工艺。 伸筋活血合剂;薄层色谱法;伸筋草;白芍;续断;甘草;定性鉴别 TLC Identi fication of Shen in Huoxue Mixture j YANG W an_hua, CHEN Bing (De t.of Pharmacy, Ruijin Hos ital of School of Medicine Affiliated to Shan hai Jiaoton Universit ,Shan hai 200025,China) g g y g XU Jia_ni(Shanghai University of Traditional Chinese M edicine, Shanghai 200032, China ) ABSTRACT OBJECT IVE:To establish a method for ualitative identification of Shen in huoxue mixture.METHODS:L _ q j y co odii a onicum , P aeonia lacti lora, D i sacus as eroides and Radix et Rhizoma Gl c rrhizae in the re aration w ere iden- p j p f p p y y tified b TLC.RES ULTS :At the corres ondin ositions, the color of TLC s ots of Paeonia lacti lora, D i sacus as eroides y g f p p and Radix et Rhizoma Gl c rrhizae in the test sam les and in reference substances w ere identical.There w as no interference y y form negative control.But no characteristic s ot s of Ly cop odii j ap onicum in the sam le were noted.CONCLUSION :The est ablished TLC identification method of P aeonia lact if lora , D ips acus asp eroides and Radix et Rhizom a Glycyrrhizae is re- roducible, s ecific, reliable and suitable for the quality control of Shenjin huoxue m ix ture.The absence of characteristic T LC s ot s of Ly cop odi