对照品法和对照提取物法测定岗松油中α-蒎烯β-蒎烯桉油精和芳樟.PDFVIP

·100· Chinese Journal of Information on TCM Oct.2016 Vol.23 No.10 对照品法和对照提取物法测定岗松油中α-蒎烯、β-蒎烯、 桉油精和芳樟醇含量比较分析 罗轶，白桂昌，陆敏仪 广西壮族自治区食品药品检验所，广西 南宁 530021 摘要：目的 分别用对照品法和对照提取物法测定岗松油中 α-蒎烯、β-蒎烯、桉油精和芳樟醇的含量，探 讨选择对照提取物替代单体对照品测定岗松油含量的可行性。方法 采用 DB-5 气相毛细管色谱柱（60 m × 0.25 mm ×0.25 µm ）；程序升温：起始温度 80 ℃，保持 15 min，1 ℃/min 升至 90 ℃，保持2 min ，10 ℃/min 升至 110 ℃，25 ℃/min 升至 240 ℃，保持 8 min；进样口温度 250 ℃；检测器温度250 ℃。结果 α-蒎烯、 β-蒎烯、桉油精和芳樟醇在各自的浓度范围内均有良好的线性关系 （r ≥0.999 ），平均回收率为 96.5% ～102.2%； t 检验表明 2 种测定方法得出的含量结果无明显差异。结论 对照提取物法可用于岗松油的质量评价。 关键词：对照品法；对照提取物法；气相色谱法；岗松油；α-蒎烯；β-蒎烯；桉油精；芳樟醇；含量测定 DOI：10.3969/j.issn.1005-5304.2016.10.023 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2016)10-0100-04 Comparative Analysis on Simultaneous Determination of α-pinene, β-pinene, Eucalyptol and Linalool in Baeckea Frutescens Preparations by Reference Substances Method and Reference Extract Method LUO Yi, BAI Gui-chang, LU Min-yi (Guangxi Institute for Food and Drug Control, Nanning 530021, China) Abstract: Objective To determinate the contents of α-pinene, β-pinene, eucalyptol and linalool in Baeckea frutescens by reference substances method and reference extract method respectively; To explore the feasibility of replacing single component reference by control extracts in assay. Methods The GC system consisted of a quartz column DB-5 (60 m ×0.25 mm ×0.25 µm); The temperature programming rose from 80 ℃ (15 min) to 90 ℃ by 1 ℃/min, lasting 2 min, then 10 ℃/min to 110 ℃, then 25 ℃/min to 240 ℃, lasting 8 min in the end; The temperature of the entrance of capillary vess