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经血源性子宫内膜间充质干细胞的分离、培养与鉴定
中国组织工程研究与临床康复彩74誉32助2010—08—06出版
JournalofClinicalRehabilitativeTissueEngineeringResearchAugust6.2010Vo1.14,No.32
经血源性子宫内膜间充质干细胞的分离,培养与鉴定冰术术米水★
周云帆,杨波,胡祥,焦洪亮.,周长辉,田毅...,雷宁静,谷晨熙.,许予I1J】,戴建武.,关方霞
Isolation,cultureandidentificationofmenstrualblood-derivedmesenchymalstemcells
ZhouYun-f叩一,
YangBo一,HuXiang一,JiaoHong.1iang一.ZhouChang.hui,
TianYi一,LeiNing-jing,GuChen---xi,XuYu—ming
DaiJian.wu.GuanFang-xia.
Departmentof
Bioangineedng,
ZhengouUnwets~ty.
Zhangzhou450001.
HenanProvince.
China;Open
LaboratoryofClinicaI
MedicineandKey
Subject,Henan
lnstitutionofHigher
EduceU~,
Zhengzhou450052.
HenanProvince.
China;Depar~entof
Neurosurge~.Firsl
AffiliatedHospita1.
~angzhouUniversity.
Zhengzhou450052
HensnProvince.
China;JiangsuPublic
TechnologySe~ce
PlatformofStemCelIs
andBio~herapy,
hou225300
JiangsuProvince.
China;.Shenzhan
BeikeCalI
EngineeringInstJtute.
Shanzhan518O00
GuangdoogProvince.
China;DDeparlmentof
CliniceIMedicine,
angzhouUniversity.
Zhangzhou45o001.
HenanProvince.
China;Deparlmentof
Neurology.
Flrstated
Hospital,Zhangzhou
Universi,Zheogzhou
450052Henan
Province.China;
lntuteofGane~cs
andDevelopmental
Biology.Chinese
AcademyofSciences.
Beijing100190.
China
ZhouJn-fan★.
Studyingformasters
degree,Departmentof
Bicengineedng.
ZhangzhouUniversity,
Zhengzhou450~1.
HenanProvince.
China;Open
LaboratoryofClinicaI
MedicineandKey
Subject,Henan
InstitutionofHigher
Education,
Z.hangzhou450052,
HenanProvince.
China
boat-1986@163.COWl
5952
Abstract
BACKGR0UND:Generally.themainsourcesofstemcellsareembryonicstemcellsandbonemarrowstemcellsbutIhereare
somelimitationsofthetwokindsofsources,andresearchersstrivetofindanewtypeofstemcellsinordertoovercomethe
potentialforteratoma,thelackofsamplesources.ethicscontroversiesandsoon.
OBJECTIVE:Toisolate,cultureandidentifymenstruaIblood—derivedmesenchymalstemcells(MMCs).
METHODS:MMCswereseparatedbvFicolIdensitygradientcentrifugation.CelImorphologyandgrowthcharacteristicswere
observed.Flowc~ometryanalysiswasusedtoidentifyex
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