经血源性子宫内膜间充质干细胞的分离、培养与鉴定.doc

经血源性子宫内膜间充质干细胞的分离、培养与鉴定 中国组织工程研究与临床康复彩74誉32助2010—08—06出版 JournalofClinicalRehabilitativeTissueEngineeringResearchAugust6.2010Vo1.14,No.32 经血源性子宫内膜间充质干细胞的分离,培养与鉴定冰术术米水★ 周云帆,杨波,胡祥,焦洪亮.,周长辉,田毅...,雷宁静,谷晨熙.,许予I1J】,戴建武.,关方霞 Isolation,cultureandidentificationofmenstrualblood-derivedmesenchymalstemcells ZhouYun-f叩一, YangBo一,HuXiang一,JiaoHong.1iang一.ZhouChang.hui, TianYi一,LeiNing-jing,GuChen---xi,XuYu—ming DaiJian.wu.GuanFang-xia. Departmentof Bioangineedng, ZhengouUnwets~ty. Zhangzhou450001. HenanProvince. China;Open LaboratoryofClinicaI MedicineandKey Subject,Henan lnstitutionofHigher EduceU~, Zhengzhou450052. HenanProvince. China;Depar~entof Neurosurge~.Firsl AffiliatedHospita1. ~angzhouUniversity. Zhengzhou450052 HensnProvince. China;JiangsuPublic TechnologySe~ce PlatformofStemCelIs andBio~herapy, hou225300 JiangsuProvince. China;.Shenzhan BeikeCalI EngineeringInstJtute. Shanzhan518O00 GuangdoogProvince. China;DDeparlmentof CliniceIMedicine, angzhouUniversity. Zhangzhou45o001. HenanProvince. China;Deparlmentof Neurology. Flrstated Hospital,Zhangzhou Universi,Zheogzhou 450052Henan Province.China; lntuteofGane~cs andDevelopmental Biology.Chinese AcademyofSciences. Beijing100190. China ZhouJn-fan★. Studyingformasters degree,Departmentof Bicengineedng. ZhangzhouUniversity, Zhengzhou450~1. HenanProvince. China;Open LaboratoryofClinicaI MedicineandKey Subject,Henan InstitutionofHigher Education, Z.hangzhou450052, HenanProvince. China boat-1986@163.COWl 5952 Abstract BACKGR0UND:Generally.themainsourcesofstemcellsareembryonicstemcellsandbonemarrowstemcellsbutIhereare somelimitationsofthetwokindsofsources,andresearchersstrivetofindanewtypeofstemcellsinordertoovercomethe potentialforteratoma,thelackofsamplesources.ethicscontroversiesandsoon. OBJECTIVE:Toisolate,cultureandidentifymenstruaIblood—derivedmesenchymalstemcells(MMCs). METHODS:MMCswereseparatedbvFicolIdensitygradientcentrifugation.CelImorphologyandgrowthcharacteristicswere observed.Flowc~ometryanalysiswasusedtoidentifyex