尿毒症患者血清对内皮细胞凋亡的影响及rhEPO的干预作用.docVIP

尿毒症患者血清对内皮细胞凋亡的影响及rhEPO的干预作用 李小丽，甘 华，常晓东，杜晓刚 （重庆医科大学附属第一医院肾内科，重庆 400016） 摘 要：目的 探讨尿毒症患者血清对人脐静脉内皮细胞(Human umbilical vein endothelial cell, HUVEC)凋亡的影响及重组人促红细胞生成素（recombinant human erythropoietin, rhEPO）的干预作用。方法 选择我院2008年12月至2009年4月收治的尿毒症患者和健康志愿者各10例。HUVEC按不同的干预方式分3组进行实验：对照组(含10％健康者血清)，尿毒症组（含10％尿毒症患者血清），rhEPO干预组（rhEPO 5、10、15 U/ml，分别加入含有10％尿毒症患者血清的培养基）。各组干预24 h，TUNEL法检测细胞凋亡，比色法测定细胞内活性氧。结果 尿毒症患者血清环境中内皮细胞的凋亡指数和活性氧水平均高于对照组(P 0.01)。rhEPO可抑制尿毒症患者血清诱导的内皮细胞凋亡(P 0.01)，降低细胞内活性氧水平(P0.01)；并且随着rhEPO浓度的增加，内皮细胞凋亡指数和ROS水平都逐渐降低(P 0.05)。相关分析显示：凋亡指数与活性氧水平呈正相关(r＝0.89，P 0.01)。结论 尿毒症患者血清可以诱导HUVEC凋亡，其机制可能与氧化应激有关。rhEPO可能通过减轻氧化应激反应而抑制尿毒症患者血清诱导的内皮细胞凋亡。 关键词：尿毒症；细胞凋亡；重组人促红细胞生成素；氧化应激 中图法分类号：R363;R979.5 文献标识码：A Effect of recombinant human erythropoietin on endothelial cell apoptosis cultured in medium containing uremic serum LI Xiao-li，GAN Hua，CHANG Xiao-dong，DU Xiao-gang，ZHOU Hong-wen（Department of Nephrology，the First Affiliated Hospital，Chongqing Medical University，Chongqing 400016，China） Abstract: Objective To explore the effect of uremic serum on cell apoptosis in hu作者简介：李小丽，女，湖北省枣阳市人，硕士研究生，主要从事慢性肾脏病方面的研究。电话：(023E-mail：lixiaoli-cuite@163.com 通信作者：甘 华，E-mail：zxg0233@收稿日期：2009-04-09；修回日期：2009-05-24man umbilical vein endothelial cells(HUVECs), and its intervention by recombinant human erythropoietin(rhEPO). Methods HUVEC were divided into 3 groups: control group(including 10% healthy serum medium), uremic group(including 10% uremic serum medium), rhEPO treatment group (rhEPO(5、10、15U/ml) was added to 10% uremic serum medium). After 24 h’s intervention, cell apoptosis was evaluated by TUNEL assay, and reactive oxygen species were detected by colorimetry. Results HUVEC apoptosis and ROS were higher in the presence of uremic serum than that of healthy serum(P0.01). With the addition of rhEPO(5,10,15U/ml) in culture medium, the percentage of apoptotic cells and the production of ROS in HUVEC were inhibited(P0.01)；and with rhEPO concentration increasing, 作者简介：李小丽，女，湖