贫营养条件下IAMBR污泥微生物群落结构的演变-中国环境科学.pdf

中国环境科学 2015,35(3) ：839~845 China Environmental Science 贫营养条件下 IAMBR 污泥微生物群落结构的演变 薛圆圆 1,孙宝盛 1*,杜 江 2,薛士琼 1,王明圆 1,李 恺 1 (1.天津大学环境科学与工程学院,天津 300072 ；2. 中国建筑设计研究院,北京 100044) 摘要：采用 PCR-DGGE 技术并结合系统处理效果研究了贫营养条件下 IAMBR 污泥微生物群落结 ,结果表明:IAMBR 污泥中总细菌多 样性特征、相似性特征和种群归属特征具有高度的协同性.运行前 18 天,氨氮去除率由 95%降至73%后增加至 82%,同时SVI 值由 123.7mL/g 升至 135.2mL/g 再降至 128.4mL/g.微生物群落在试验末期演替剧烈,总细菌相似性指数下降到 63.6%,SVI 值最终升至 132.5mL/g.通过克隆 测序分析,IAMBR 系统中微生物菌种大部分为未培养菌种,其中亚硝化螺菌属占据优势地位,说明贫营养环境对 IAMBR 微生物群落产生不 良影响,污泥微生物功能性指向明显,即硝化功能菌占据优势地位. 关键词：贫营养；IAMBR ；PCR-DGGE ；活性污泥；微生物群落结 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2015)03-0839-07 Analysis of IAMBR on succession of sludge microbial community composition in the oligotrophic condition. XUE 1 1* 2 1 1 1 Yuan-yuan , SUN Bao-sheng , DU Jiang , XUE Shi-qiong , WANG Ming-yuan , LI Kai (1.School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China；2.China Architecture Design and Research Group, Beijing 100044, China). China Environmental Science, 2015,35(3)：839~845 Abstract ：The microbial community in the IAMBR activated sludge grown in a oligotrophic condition was investigated with PCR-DGGE technology and the treatment effect of the IAMBR system was evaluated. The results showed that there was a high degree of cooperativity among the diversity, similarity and population belonging of the total bacteria. In the first 18days, the ammonia nitrogen removal dropped from 95% to 73%, and then increased to 82%. At the same time, the SVI increased from 123.7mL/g to 135.2mL/g, and then decreased to 128.4mL/g. Microbial community experienced a drastic succession at the end of experimental period. The similarity index of bacteria dropped to 63.6%, and the SVI increa